An Agrobacterium rhizogenes mediated hairy root transformation protocol for fenugreek.
MethodsX
; 7: 101098, 2020.
Article
en En
| MEDLINE
| ID: mdl-33102159
ABSTRACT
â¢This work describes a protocol for hairy root transformation of the medicinal crop legume fenugreek (Trigonella foenum-graecum L.). Hairy root plant transformation mediated by Agrobacterium rhizogenes is an established method for the rapid genetic transformation of various dicotyledonous plants. We have adapted a hairy root transformation protocol from the model legume Medicago truncatula for use in this metabolically rich non-model crop legume. Considering the great variety and abundance of phytochemicals in fenugreek and its established use in traditional medicine, we aim for this method to become a resource for metabolic pathway identification and for production of valuable specialised metabolites via metabolic engineering approaches.â¢Development rapid transformation (2.5-3 weeks) of fenugreek roots via A. rhizogenes.â¢Marker gene cassette with suitable promoter for visual detection of transformed fenugreek roots.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
MethodsX
Año:
2020
Tipo del documento:
Article
País de afiliación:
Grecia