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Development and Expression of Subunit Vaccines Against Viruses in Plants.
Esqueda, Adrian; Chen, Qiang.
Afiliación
  • Esqueda A; The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ, USA.
  • Chen Q; The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ, USA. Qiang.Chen.4@asu.edu.
Methods Mol Biol ; 2225: 25-38, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33108655
ABSTRACT
Various systems exist for the robust production of recombinant proteins. However, only a few systems are optimal for human vaccine protein production. Plant-based transient protein expression systems offer an advantageous alternative to costly mammalian cell culture-based systems and can perform posttranslational modifications due to the presence of an endomembrane system that is largely similar to that of the animal cell. Technological advances in expression vectors for transient expression in the last two decades have produced new plant expression systems with the flexibility and speed that cannot be matched by those based on mammalian or insect cell culture. The rapid and high-level protein production capability of transient expression systems makes them the optimal system to quickly and versatilely develop and produce vaccines against viruses such as 2019-nCoV that have sudden and unpredictable outbreaks. Here, expression of antiviral subunit vaccines in Nicotiana benthamiana plants via transient expression is demonstrated.
Asunto(s)
Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Plantas / Neumonía Viral / Infecciones por Coronavirus / Vacunas de Subunidad / Pandemias / Betacoronavirus Límite: Humanos Idioma: Inglés Revista: Methods Mol Biol Asunto de la revista: Biologia Molecular Año: 2021 Tipo del documento: Artículo País de afiliación: Estados Unidos

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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Plantas / Neumonía Viral / Infecciones por Coronavirus / Vacunas de Subunidad / Pandemias / Betacoronavirus Límite: Humanos Idioma: Inglés Revista: Methods Mol Biol Asunto de la revista: Biologia Molecular Año: 2021 Tipo del documento: Artículo País de afiliación: Estados Unidos