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Modelling early events in Mycobacterium bovis infection using a co-culture model of the bovine alveolus.
Lee, Diane Frances; Stewart, Graham Roger; Chambers, Mark Andrew.
Afiliación
  • Lee DF; School of Veterinary Medicine, University of Surrey, Guildford, Surrey, UK. diane.lee@surrey.ac.uk.
  • Stewart GR; School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, UK.
  • Chambers MA; School of Veterinary Medicine, University of Surrey, Guildford, Surrey, UK.
Sci Rep ; 10(1): 18495, 2020 10 28.
Article en En | MEDLINE | ID: mdl-33116165
ABSTRACT
Bovine tuberculosis (bTB), a zoonosis mainly caused by Mycobacterium bovis has severe socio-economic consequences and impact on animal health. Host-pathogen interactions during M. bovis infection are poorly understood, especially early events which are difficult to follow in vivo. This study describes the utilisation of an in vitro co-culture model, comprising immortalised bovine alveolar type II (BATII) epithelial cells and bovine pulmonary arterial endothelial cells (BPAECs). When cultured at air-liquid interface, it was possible to follow the migration of live M. bovis Bacille Calmette-Guérin (BCG) and to observe interactions with each cell type, alongside cytokine release. Infection with BCG was shown to exert a detrimental effect primarily upon epithelial cells, with corresponding increases in IL8, TNFα, IL22 and IL17a cytokine release, quantified by ELISA. BCG infection increased expression of CD54, MHC Class I and II molecules in endothelial but not epithelial cells, which exhibited constitutive expression. The effect of peripheral blood mononuclear cell conditioned medium from vaccinated cattle upon apical-basolateral migration of BCG was examined by quantifying recovered BCG from the apical, membrane and basolateral fractions over time. The numbers of recovered BCG in each fraction were unaffected by the presence of PBMC conditioned medium, with no observable differences between vaccinated and naïve animals.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alveolos Pulmonares / Tuberculosis Bovina / Vacuna BCG / Células Endoteliales / Células Epiteliales Alveolares / Enfermedades Pulmonares Límite: Animals Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alveolos Pulmonares / Tuberculosis Bovina / Vacuna BCG / Células Endoteliales / Células Epiteliales Alveolares / Enfermedades Pulmonares Límite: Animals Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido