Development and validation of HPLC method for simultaneous quantification of alpha-tocopherol (free or encapsulated) and cholesterol in semen cryopreservation media.
Biomed Chromatogr
; 35(4): e5018, 2021 Apr.
Article
en En
| MEDLINE
| ID: mdl-33125786
ABSTRACT
The HPLC method was developed and validated for assaying alpha-tocopherol and cholesterol in cryopreservation media. Chromatographic separation was performed on an isocratic system, using a C-18 column. The mobile phase was composed of a mixture of methanolacetonitrilewater 68284 (v/v/v), using a flow rate of 1.5 mL/min and 20 µL injection volume, at a wavelength of 208 nm. The method was validated according to International Conference on Harmonization guidelines. The method proved to be specific, accurate, precise, and linear with correlation coefficients greater than 0.996 over a wide concentration range of both analytes. Vitamin E and cholesterol presented limits of detection of 0.002 mg/mL, 0.026 mg/mL and limits of quantitation of 0.006 mg/mL, 0.086 mg/mL, respectively. This method is simple and rapid, shows high precision and accuracy, and offers the advantage of simultaneous assaying of vitamin E and cholesterol (alone, in cyclodextrins complexes or in liposome loaded) on semen cryopreservation media.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Preservación de Semen
/
Criopreservación
/
Colesterol
/
Soluciones Preservantes de Órganos
/
Alfa-Tocoferol
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biomed Chromatogr
Año:
2021
Tipo del documento:
Article
País de afiliación:
Argelia