Fucoidan isolated from Hizikia fusiforme suppresses ultraviolet B-induced photodamage by down-regulating the expressions of matrix metalloproteinases and pro-inflammatory cytokines via inhibiting NF-κB, AP-1, and MAPK signaling pathways.

ABSTRACT

Overexposure to ultraviolet B (UVB) causes skin damage. The purpose of this study was to evaluate the protective effect of a fucoidan with a molecular weight of 102.67 kDa, isolated from Hizikia fusiforme, against UVB-induced photodamage in vitro in human dermal fibroblasts (HDFs) and in vivo in zebrafish. Fucoidan remarkably inhibited commercial collagenase. Additionally, it significantly and dose-dependently decreased the intracellular reactive oxygen species (ROS) levels and increased the viability of UVB-irradiated HDFs. Furthermore, fucoidan remarkably improved collagen synthesis, inhibited intracellular collagenase, and reduced the expression of matrix metalloproteinases and pro-inflammatory cytokines in UVB-irradiated HDFs. Further research demonstrated that these effects occurred through the regulation of the activator protein 1, nuclear factor kappa B, and mitogen-activated protein kinase signaling pathways. Furthermore, the in vivo results showed that fucoidan protected zebrafish larvae against UVB-induced photodamage by decreasing cell death via the suppression of lipid peroxidation and inflammatory response through ROS clearance. In conclusion, fucoidan isolated from Hizikia fusiforme exhibits strong in vitro and in vivo photoprotective effects, and can be used as an ingredient in the cosmeceutical and pharmaceutical industries.