A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activation.
Cell
; 51(5): 709-19, 1987 Dec 04.
Article
en En
| MEDLINE
| ID: mdl-3315230
ABSTRACT
Repression of the yeast silent mating type loci requires cis-acting sequences located over 1 kb from the regulated promoters. One of these sites, a "silencer," exhibits enhancer-like distance- and orientation-independence. The silencer demonstrates both autonomous replication sequence (ARS) activity and a centromere-like segregation function, suggesting roles for DNA replication and segregation in transcriptional repression. Here we identify three sequences (A, E, and B) involved both in repression and in either ARS or segregation activity. The sequences are functionally redundant no one is essential for complete transcriptional control, but mutations in any two inactivate the silencer. Surprisingly, elements E and B can each activate transcription from heterologous promoters, and E shows striking homology to several yeast upstream activation sequences. Therefore, sequences individually involved in replication, segregation, and transcriptional activation can, at the silencer, efficiently repress transcription.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Saccharomyces cerevisiae
/
Transcripción Genética
/
ADN de Hongos
/
Genes Reguladores
/
Replicación del ADN
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Cell
Año:
1987
Tipo del documento:
Article
País de afiliación:
Reino Unido