Your browser doesn't support javascript.
loading
CLP gene family, a new gene family of Cotesia vestalis bracovirus inhibits melanization of Plutella xylostella hemolymph.
Wang, Ze-Hua; Zhou, Yue-Nan; Ye, Xi-Qian; Wu, Xiao-Tong; Yang, Pei; Shi, Min; Huang, Jian-Hua; Chen, Xue-Xin.
Afiliación
  • Wang ZH; Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
  • Zhou YN; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China.
  • Ye XQ; Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
  • Wu XT; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China.
  • Yang P; Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
  • Shi M; Zhejiang Provincial Key Lab of Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China.
  • Huang JH; Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
  • Chen XX; Zhejiang Provincial Key Lab of Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China.
Insect Sci ; 28(6): 1567-1581, 2021 Dec.
Article en En | MEDLINE | ID: mdl-33155403
ABSTRACT
Polydnaviruses (PDVs) are obligatory symbionts of parasitoid wasps and play an important role in suppressing host immune defenses. Although PDV genes that inhibit host melanization are known in Microplitis bracovirus, the functional homologs in Cotesia bracoviruses remain unknown. Here, we find that Cotesia vestalis bracovirus (CvBV) can inhibit hemolymph melanization of its host, Plutella xylostella larvae, during the early stages of parasitization, and that overexpression of highly expressed CvBV genes reduced host phenoloxidase activity. Furthermore, CvBV-7-1 in particular reduced host phenoloxidase activity within 12 h, and the injection of anti-CvBV-7-1 antibody increased the melanization of parasitized host larvae. Further analyses showed that CvBV-7-1 and three homologs from other Cotesia bracoviruses possessed a C-terminal leucine/isoleucine-rich region and had a similar function in inhibiting melanization. Therefore, a new family of bracovirus genes was proposed and named as C-terminal Leucine/isoleucine-rich Protein (CLP). Ectopic expression of CvBV-7-1 in Drosophila hemocytes increased susceptibility to bacterial repression of melanization and reduced the melanotic encapsulation of parasitized D. melanogaster by the parasitoid Leptopilina boulardi. The formation rate of wasp pupae and the eclosion rate of C. vestalis were affected when the function of CvBV-7-1 was blocked. Our findings suggest that CLP genes from Cotesia bracoviruses encoded proteins that contain a C-terminal leucine/isoleucine-rich region and function as melanization inhibitors during the early stage of parasitization, which is important for successful parasitization.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pigmentación / Polydnaviridae / Genes Virales / Melaninas / Mariposas Nocturnas Límite: Animals Idioma: En Revista: Insect Sci Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pigmentación / Polydnaviridae / Genes Virales / Melaninas / Mariposas Nocturnas Límite: Animals Idioma: En Revista: Insect Sci Año: 2021 Tipo del documento: Article País de afiliación: China