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Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System.
Morales-Mantilla, Daniel E; Huang, Xinyan; Erice, Philip; Porter, Paul; Zhang, Yun; Figueroa, Mary; Chandra, Joya; King, Katherine Y; Kheradmand, Farrah; Rodríguez, Antony.
Afiliación
  • Morales-Mantilla DE; Program in Immunology, Baylor College of Medicine; Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine.
  • Huang X; Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine; Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University.
  • Erice P; Program in Immunology, Baylor College of Medicine; Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine.
  • Porter P; Department of Medicine, Pulmonary, Critical Care, Sleep Medicine, Baylor College of Medicine.
  • Zhang Y; Program in Immunology, Baylor College of Medicine; Department of Pathology and Immunology, Baylor College of Medicine.
  • Figueroa M; Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center.
  • Chandra J; Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center.
  • King KY; Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine.
  • Kheradmand F; Department of Medicine, Pulmonary, Critical Care, Sleep Medicine, Baylor College of Medicine; Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center.
  • Rodríguez A; Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine; Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center; antonyr@bcm.edu.
J Vis Exp ; (164)2020 10 22.
Artículo en Inglés | MEDLINE | ID: mdl-33165327
ABSTRACT
Close to 14% of adults in the United States were reported to smoke cigarettes in 2018. The effects of cigarette smoke (CS) on lungs and cardiovascular diseases have been widely studied, however, the impact of CS in other tissues and organs such as blood and bone marrow remain incompletely defined. Finding the appropriate system to study the effects of CS in rodents can be prohibitively expensive and require the purchase of commercially available systems. Thus, we set out to build an affordable, reliable, and versatile system to study the pathologic effects of CS in mice. This whole-body inhalation exposure system (WBIS) set-up mimics the breathing and puffing of cigarettes by alternating exposure to CS and clean air. Here we show that this do-it-yourself (DIY) system induces airway inflammation and lung emphysema in mice after 4-months of cigarette smoke exposure. The effects of whole-body inhalation (WBI) of CS on hematopoietic stem and progenitor cells (HSPCs) in the bone marrow using this apparatus are also shown.
Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Tipo de estudio: Estudio pronóstico Idioma: Inglés Año: 2020 Tipo del documento: Artículo

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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Tipo de estudio: Estudio pronóstico Idioma: Inglés Año: 2020 Tipo del documento: Artículo
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