Polysaccharides from Pleurotus eryngii: Selective extraction methodologies and their modulatory effects on THP-1 macrophages.
Carbohydr Polym
; 252: 117177, 2021 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-33183624
Polysaccharides from P. eryngii mushroom were selectively extracted using low-cost technologies (water at different conditions of temperature and pressure). Mannogalactan was the main polysaccharide in cold-water extracted fraction (CWEF), while a linear (1â6)-ß-d-glucan was the main polymer in hot-water extracted fraction (HWEF). Autoclave-extracted fraction (AEF) contained a mixture of at least four different α- and ß-glucans. The report of linear (1â6)-ß-glucan and linear (1â3)-ß-glucan is a new finding for P. eryngii fruiting bodies. The immunostimulatory properties of the fractions on THP-1 macrophages were studied. All fractions at 50, 250 and 500 µg/mL were not cytotoxic and produced different stimulus on NO, IL-1ß and IL-10 secretion by the cells. Thus, our results showed that it is possible to concentrate different P. eryngii polysaccharides in selected fractions using a simple and low-cost procedure. Since biological effects depends on the polysaccharide structure, this technique allows the obtainment of fractions with distinct immunomodulatory activities.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Pleurotus
/
Cuerpos Fructíferos de los Hongos
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Beta-Glucanos
/
Polisacáridos Fúngicos
/
Factores Inmunológicos
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Macrófagos
Límite:
Humans
Idioma:
En
Revista:
Carbohydr Polym
Año:
2021
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Reino Unido