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Deciphering the Aldolase Function of STM3780 from a Bovine Enteric Infection-Related Gene Cluster in Salmonella enterica Serotype Typhimurium.
Zhi, Yuan; Xiang, Dao Feng; Narindoshvili, Tamari; Andrews-Polymenis, Helene; Raushel, Frank M.
Afiliación
  • Zhi Y; Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843, United States.
  • Xiang DF; Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
  • Narindoshvili T; Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
  • Andrews-Polymenis H; Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M University College of Medicine, Bryan, Texas 77807, United States.
  • Raushel FM; Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843, United States.
Biochemistry ; 59(48): 4573-4580, 2020 12 08.
Article en En | MEDLINE | ID: mdl-33231431
Non-typhoidal Salmonella are capable of colonizing livestock and humans, where they can progressively cause disease. Previously, a library of targeted single-gene deletion mutants of Salmonella enterica serotype Typhimurium was inoculated to ligated ileal loops in calves to identify genes under selection. Of those genes identified, a cluster of genes is related to carbohydrate metabolism and transportation. It is proposed that an incoming carbohydrate is first phosphorylated by a phosphoenolpyruvate-dependent phosphotransferase system. The metabolite is further phosphorylated by the kinase STM3781 and then cleaved by the aldolase STM3780. STM3780 is functionally annotated as a class II fructose-bisphosphate aldolase. The aldolase was purified to homogeneity, and its aldol condensation activity with a range of aldehydes was determined. In the condensation reaction, STM3780 was shown to catalyze the abstraction of the pro-S hydrogen from C3 of dihydroxyacetone and subsequent formation of a carbon-carbon bond with S stereochemistry at C3 and R stereochemistry at C4. The best aldehyde substrate was identified as l-threouronate. Surprisingly, STM3780 was also shown to catalyze the condensation of two molecules of dihydroxyacetone phosphate to form the branched carbohydrate dendroketose bisphosphate.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella typhimurium / Proteínas Bacterianas / Fructosa-Bifosfato Aldolasa / Genes Bacterianos Límite: Animals / Humans Idioma: En Revista: Biochemistry Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella typhimurium / Proteínas Bacterianas / Fructosa-Bifosfato Aldolasa / Genes Bacterianos Límite: Animals / Humans Idioma: En Revista: Biochemistry Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos