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Apolipoprotein A4 regulates the immune response in carbon tetrachloride-induced chronic liver injury in mice.
Wang, Yinan; Yang, Ziyu; Wei, Yang; Li, Xiaoming; Li, Shengbin.
Afiliación
  • Wang Y; Bio-evidence Science Academy (BSA), Xi'an Jiaotong University (XJTU), No. 205 Zhuque Street, Xi'an 710061, China; Key laboratory of Ministry of Public Health for Forensic Sciences, No. 205 Zhuque Street, Xi'an 710061, China.
  • Yang Z; Bio-evidence Science Academy (BSA), Xi'an Jiaotong University (XJTU), No. 205 Zhuque Street, Xi'an 710061, China; Key laboratory of Ministry of Public Health for Forensic Sciences, No. 205 Zhuque Street, Xi'an 710061, China.
  • Wei Y; National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, Precision Medical Institute, Institute of Digestive Diseases. The Second Affiliated Hospital, Xi'an Jiaotong University, No. 157 West 5th Road, Xi'an 710004, China.
  • Li X; National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, Precision Medical Institute, Institute of Digestive Diseases. The Second Affiliated Hospital, Xi'an Jiaotong University, No. 157 West 5th Road, Xi'an 710004, China. Electronic address: lixiaoming022916@163.com.
  • Li S; Bio-evidence Science Academy (BSA), Xi'an Jiaotong University (XJTU), No. 205 Zhuque Street, Xi'an 710061, China; Key laboratory of Ministry of Public Health for Forensic Sciences, No. 205 Zhuque Street, Xi'an 710061, China. Electronic address: shengbin_li19@163.com.
Int Immunopharmacol ; 90: 107222, 2021 Jan.
Article en En | MEDLINE | ID: mdl-33276196
ABSTRACT
This article explores the role of ApoA4 in a CCl4-induced chronic liver injury (CLI) mouse model. C57BL/6J mice (WT) and ApoA4 knock-out (KO) mice were divided into CCl4 CLI (WT-CCl4 and KO-CCl4) and olive oil solvent control groups (WT-Veh and KO-Veh). Some of the KO-CCl4 mice were additionally treated with recombinant mouse ApoA4 and primary mouse T lymphocyte injections. After 6 weeks, histological analyses, biochemical and superoxide dismutase (SOD) and malondialdehyde (MDA) assays, flow cytometry of immune cells and qRT-PCR analyses were performed. KO mice after treatment with CCl4 showed reduced hepatic SOD and enhanced serum MDA activities leading to worsening liver injury and fibrosis compared with WT-CCl4, accompanied by enhanced hepatic alpha smooth muscle actin (α-SMA), tissue inhibitor of metalloproteinases-1 (TIMP-1) and collagen type I alpha 1 chain (COL1A1) transcriptions, elevated macrophage M1 levels, enhanced tumor necrosis factor-alpha (TNF-α), Interleukin 6 (IL-6) and C-C Motif Chemokine Ligand 5 (CCL5), but reduced Interleukin 10 (IL-10), monocyte chemotactic protein 1 (MCP-1), C-C Motif Chemokine Receptor 2 (CCR2), C-X3-C Motif Chemokine Receptor 1 (CX3CR1) and C-X-C Motif Chemokine Ligand 9 (CXCL9) transcription, as well as reduced CD3+, CD4+ and CD8+ T cell percentages in hepatic tissue, blood cells and spleen. In addition, CD11b+CD115+, CD11b+/Ly6Chigh, CD11b+/LyC6- and CD11b+/Ly6Cint cells were enhanced, which partly reversed by ApoA4 protein and T cell injections. In conclusion, we propose that ApoA4 might be involved in liver protection via inhibiting fibrotic mediators and inflammatory cytokines, suppression of pro-inflammatory hepatic M1 cell invasion and regulation of CD8+ T and CD4+ T lymphocytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apolipoproteínas A / Citocinas / Mediadores de Inflamación / Enfermedad Hepática Crónica Inducida por Sustancias y Drogas / Cirrosis Hepática Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int Immunopharmacol Asunto de la revista: ALERGIA E IMUNOLOGIA / FARMACOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apolipoproteínas A / Citocinas / Mediadores de Inflamación / Enfermedad Hepática Crónica Inducida por Sustancias y Drogas / Cirrosis Hepática Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int Immunopharmacol Asunto de la revista: ALERGIA E IMUNOLOGIA / FARMACOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China
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