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Abiotic stressors impact outer membrane vesicle composition in a beneficial rhizobacterium: Raman spectroscopy characterization.
Potter, Matthew; Hanson, Cynthia; Anderson, Anne J; Vargis, Elizabeth; Britt, David W.
Afiliación
  • Potter M; Department of Biological Engineering, Utah State University, Logan, UT, 84322, USA.
  • Hanson C; Department of Biological Engineering, Utah State University, Logan, UT, 84322, USA.
  • Anderson AJ; Department of Biological Engineering, Utah State University, Logan, UT, 84322, USA.
  • Vargis E; Department of Biological Engineering, Utah State University, Logan, UT, 84322, USA. vargis@usu.edu.
  • Britt DW; Department of Biological Engineering, Utah State University, Logan, UT, 84322, USA. david.britt@usu.edu.
Sci Rep ; 10(1): 21289, 2020 12 04.
Article en En | MEDLINE | ID: mdl-33277560
ABSTRACT
Outer membrane vesicles (OMVs) produced by Gram-negative bacteria have roles in cell-to-cell signaling, biofilm formation, and stress responses. Here, the effects of abiotic stressors on OMV contents and composition from biofilm cells of the plant health-promoting bacterium Pseudomonas chlororaphis O6 (PcO6) are examined. Two stressors relevant to this root-colonizing bacterium were examined CuO nanoparticles (NPs)-a potential fertilizer and fungicide- and H2O2-released from roots during plant stress responses. Atomic force microscopy revealed 40-300 nm diameter OMVs from control and stressed biofilm cells. Raman spectroscopy with linear discriminant analysis (LDA) was used to identify changes in chemical profiles of PcO6 cells and resultant OMVs according to the cellular stressor with 84.7% and 83.3% accuracies, respectively. All OMVs had higher relative concentrations of proteins, lipids, and nucleic acids than PcO6 cells. The nucleic acid concentration in OMVs exhibited a cellular stressor-dependent increase CuO NP-induced OMVs > H2O2-induced OMVs > control OMVs. Biochemical assays confirmed the presence of lipopolysaccharides, nucleic acids, and protein in OMVs; however, these assays did not discriminate OMV composition according to the cellular stressor. These results demonstrate the sensitivity of Raman spectroscopy using LDA to characterize and distinguish cellular stress effects on OMVs composition and contents.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Vesículas Extracelulares / Pseudomonas chlororaphis / Membrana Externa Bacteriana Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Vesículas Extracelulares / Pseudomonas chlororaphis / Membrana Externa Bacteriana Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos