circARRDC3 contributes to interleukin13induced inflammatory cytokine and mucus production in nasal epithelial cells via the miR375/KLF4 axis.
Mol Med Rep
; 23(2)2021 02.
Article
en En
| MEDLINE
| ID: mdl-33313951
ABSTRACT
Allergic rhinitis (AR) is a common inflammatory disorder of the nasal mucosa. It is a major risk factor for asthma development, and uncontrolled AR can lead to the worsening of asthma symptoms, which affects the quality of life and productivity of patients. Circular RNAs (circRNA) were reported to be involved in the pathogenesis of AR. The aim of the present study was to investigate the functional role of circRNA arrestin domaincontaining 3 (circARRDC3) in AR progression. circARRDC3 knockdown suppressed the levels of granulocytemacrophage colonystimulating factor (GMCSF) and eotaxin and mucin 5AC (MUC5AC) in IL13induced nasal epithelial cells. Moreover, circARRDC3 silencing promoted viability and suppressed apoptosis in IL13induced NECs. circARRDC3 targeted microRNA (miR)375 and negatively regulated its expression. miR375 inhibition reversed the effects of circARRDC3 knockdown on GMCSF, eotaxin and MUC5AC expression levels, cell viability and cell apoptosis. In addition, miR375 inhibited krueppellike factor 4 (KLF4) expression through direct interaction, and miR375 overexpression inhibited GMCSF, eotaxin and MUC5AC expression levels, and cell apoptosis, which was abolished following KLF4 overexpression. In addition, circARRDC3, miR375 and KLF4 were all dysregulated in the nasal mucosa of patients with AR. miR375 expression was negatively correlated with circARRDC3 and KLF4 expression, and circARRDC3 expression was positively correlated with KLF4 expression. In conclusion, circARRDC3 contributed to the development of AR by regulating the miR375/KLF4 axis. These findings may provide novel insights into the pathogenesis of AR.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arrestinas
/
MicroARNs
/
Factores de Transcripción de Tipo Kruppel
/
Rinitis Alérgica
/
ARN Circular
/
Mucosa Nasal
Tipo de estudio:
Risk_factors_studies
Aspecto:
Patient_preference
Límite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Mol Med Rep
Año:
2021
Tipo del documento:
Article