Co-immobilization of multi-enzyme on reversibly soluble polymers in cascade catalysis for the one-pot conversion of gluconic acid from corn straw.
Bioresour Technol
; 321: 124509, 2021 Feb.
Article
en En
| MEDLINE
| ID: mdl-33316703
ABSTRACT
The difficulties in the process of cellulose cascade conversion based on immobilization technology lies in the recycling enzymes from rich solid-containing straw hydrolysate and the incompatibility of conventional immobilization with this process. In this study, three types of enzyme (cellulase, glucose oxidase and catalase) were successfully immobilized on a reversible soluble Eudragit L-100. Through the determination of the preparation conditions, enzymatic properties and catalytic conditions, the co-immobilized enzyme was applied to the catalytic reaction of one-pot conversion of corn straw to gluconic acid. The yield of gluconic acid achieved 0.28 mg/mg, conversion rate of cellulose in corn straw to gluconic acid reached 61.41%. The recovery of co-immobilized enzyme from solid substrate was achieved by using reversible and soluble characteristics of the carrier. After 6 times of recycling, the activity of co-immobilized enzyme was maintained at 52.38%, confirming the feasibility of multi-enzyme immobilization strategy using reversible soluble carrier in cascade reactions.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Zea mays
Idioma:
En
Revista:
Bioresour Technol
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2021
Tipo del documento:
Article
País de afiliación:
China