RNA-Centric Methods: Toward the Interactome of Specific RNA Transcripts.
Trends Biotechnol
; 39(9): 890-900, 2021 09.
Article
en En
| MEDLINE
| ID: mdl-33353763
ABSTRACT
RNA-protein interactions play an important role in numerous cellular processes in health and disease. In recent years, the global RNA-bound proteome has been extensively studied, uncovering many previously unknown RNA-binding proteins. However, little is known about which particular proteins bind to which specific RNA transcript. In this review, we provide an overview of methods to identify RNA-protein interactions, with a particular focus on strategies that provide insights into the interactome of specific RNA transcripts. Finally, we discuss challenges and future directions, including the potential of CRISPR-RNA targeting systems to investigate endogenous RNA-protein interactions.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN
/
Proteínas de Unión al ARN
/
Biología Computacional
Idioma:
En
Revista:
Trends Biotechnol
Año:
2021
Tipo del documento:
Article
País de afiliación:
Países Bajos