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The specific effect of (R)-(+)-pulegone on growth and biofilm formation in multi-drug resistant Escherichia coli and molecular mechanisms underlying the expression of pgaABCD genes.
Gong, Haiyan; He, Lijuan; Zhao, Zhilong; Mao, Xinmin; Zhang, Chen.
Afiliación
  • Gong H; The Fifth Affiliated Hospital of Xinjiang Medical University, Xinshi District, No. 118 Henan West Road, Urumqi, Xinjiang, PR China. Electronic address: gonghaiyan1217@sina.com.
  • He L; College of Public Health of Xinjiang Medical University, Shuimogou District, No. 567 Shangde North Road, Urumqi, Xinjiang, PR China. Electronic address: 964345951@qq.com.
  • Zhao Z; The Fifth Affiliated Hospital of Xinjiang Medical University, Xinshi District, No. 118 Henan West Road, Urumqi, Xinjiang, PR China. Electronic address: 670914993@qq.com.
  • Mao X; College of Traditional Chinese Medicine, Key Discipline of Integrated Traditional Chinese and Western Medicine of Autonomous Region from Xinjiang Medical University, Shuimogou District, No. 567 Shangde North Road, Urumqi, Xinjiang, PR China. Electronic address: 178782513@qq.com.
  • Zhang C; Xinjiang Medical University, Shuimogou District, No. 567 Shangde North Road, Urumqi, Xinjiang, PR China. Electronic address: nuanxintianshi@126.com.
Biomed Pharmacother ; 134: 111149, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33385683
ABSTRACT
E. coli is associated with high rates of infection and resistance to drugs not only in China but also the rest of the world. In addition, the number of E. coli biofilm infections continue to increase with time. Notably, biofilms are attractive targets for the prevention of infections caused by multidrug-resistant bacteria. Moreover, the pgaABCD-encoded Poly-ß-1,6-N-acetyl-d-glucosamine (PNAG) plays an important role in biofilm formation. Therefore, this study aimed to explore the specific effect of the (R)-(+)-pulegone (PU) on growth and biofilm formation in multi-drug resistant E. coli. The molecular mechanisms involved were also examined. The results showed that PU had significant antibacterial and antibiofilm formation activity against E. coli K1, with MIC and MBC values of 23.68 and 47.35 mg/mL, respectively. On the other hand, the maximum inhibition rate for biofilm formation in the bacterium was 52.36 % at 94.70 mg/mL of PU. qRT-PCR data showed that PU significantly down-regulated expression of the pgaABCD genes (P < 0.05). PU was also broadly effective against biofilm formation in MG1655 and MG1655/ΔpgaABCD, exhibiting the maximum inhibition rates were 98.23 % and 93.35 %, respectively. In addition, PU destroyed pre-formed mature biofilm in both MG1655 and MG1655/ΔpgaABCD about 95.03 % and 92.4 %, respectively. The study therefore verified that pgaA was a potential and key target for PU in E. coli although it was not the only one. Overall, the findings indicated that PU is a potential and novel inhibitor of drug resistance, This therefore gives insights on new ways of preventing and treating biofilm-associated infections in the food industry as well as in clinical practice.
Asunto(s)
Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Biopelículas / Proteínas de Escherichia coli / Farmacorresistencia Bacteriana Múltiple / Escherichia coli K12 / Monoterpenos Ciclohexánicos / Antibacterianos Idioma: Inglés Revista: Biomed Pharmacother Año: 2021 Tipo del documento: Artículo

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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Biopelículas / Proteínas de Escherichia coli / Farmacorresistencia Bacteriana Múltiple / Escherichia coli K12 / Monoterpenos Ciclohexánicos / Antibacterianos Idioma: Inglés Revista: Biomed Pharmacother Año: 2021 Tipo del documento: Artículo
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