Monoclonal Antibody HlyIIC­15 to C-End Domain HlyII <i>B. cereus</i> Interacts with the Trombin Recognition Site.

Zamyatina, A V; Rudenko, N V; Karatovskaya, A P; Shepelyakovskaya, A O; Siunov, A V; Andreeva-Kovalevskaya, Zh I; Nagel, A S; Salyamov, V I; Kolesnikov, A S; Brovko, F A; Solonin, A S

Monoclonal Antibody HlyIIC15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site.

Zamyatina, A V; Rudenko, N V; Karatovskaya, A P; Shepelyakovskaya, A O; Siunov, A V; Andreeva-Kovalevskaya, Zh I; Nagel, A S; Salyamov, V I; Kolesnikov, A S; Brovko, F A; Solonin, A S.

Afiliación

Zamyatina AV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Rudenko NV; Pushchino State Natural Science Institute, Pushchino, Moscow oblast 142290 Russia.
Karatovskaya AP; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Shepelyakovskaya AO; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Siunov AV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Andreeva-Kovalevskaya ZI; Scryabin Institute of Biochemistry and Physiology of Microorganisms, Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Nagel AS; Scryabin Institute of Biochemistry and Physiology of Microorganisms, Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Salyamov VI; Scryabin Institute of Biochemistry and Physiology of Microorganisms, Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Kolesnikov AS; Scryabin Institute of Biochemistry and Physiology of Microorganisms, Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Brovko FA; Scryabin Institute of Biochemistry and Physiology of Microorganisms, Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.
Solonin AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast 142290 Russia.

Russ J Bioorg Chem ; 46(6): 1214-1220, 2020.

Article en En | MEDLINE | ID: mdl-33390685

ABSTRACT

ABSTRACT

Among the panel of monoclonal antibodies to the recombinant protein HlyIICTD Bacillus cereus an antibody was found capable of forming an immune complex with a thrombin recognition region, the amino acid sequence of which is located inside the recombinant HlyIICTD. Localization of the epitope was carried out using peptide phage display methods, as well as enzyme immunoassay and immunoblotting for interaction with recombinant proteins, either containing or not containing individual components HlyIICTD. The identified epitope is located in the region of the thrombin site and retains the ability to interact with the antibody after the proteolyotic attack of the protein by thrombin.

Palabras clave

Bacillus cereus sensu lato; bacterial pore-forming toxins; epitope; fusion recombinant proteins; monoclonal antibodies; phage display; thrombin

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Russ J Bioorg Chem Año: 2020 Tipo del documento: Article