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LncRNA loc105377478 promotes NPs-Nd2O3-induced inflammation in human bronchial epithelial cells through the ADIPOR1/NF-κB axis.
Yu, Feng; Zhang, Xia; Gao, Lei; Xue, Hainan; Liu, Ling; Wang, Suhua; Chen, Shijie; Huang, Lihua.
Afiliación
  • Yu F; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China.
  • Zhang X; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China.
  • Gao L; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China.
  • Xue H; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China.
  • Liu L; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China.
  • Wang S; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China.
  • Chen S; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China.
  • Huang L; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China. Electronic address: huanglihua@btmc.edu.cn.
Ecotoxicol Environ Saf ; 208: 111609, 2021 Jan 15.
Article en En | MEDLINE | ID: mdl-33396129
With the wide application of neodymium oxide nanoparticles (NPs-Nd2O3) in various fields, their health hazards have aroused public concern in recent years. However, data regarding the cytotoxicity of NPs-Nd2O3 is limited. In this study, we investigated the function and mechanism of long-chain non-coding RNAs (lncRNAs) in NPs-Nd2O3-induced airway inflammation. Treatment with NPs-Nd2O3 induced an inflammatory response in human bronchial epithelial cells (16HBE) by upregulating the expression of interleukin-6 (IL-6) and interleukin-8 (IL-8). The levels of LDH and intracellular ROS in the cells treated by various doses of NPs-Nd2O3 also increased significantly. After treatment with 10 µg/ml NPs-Nd2O3, RNA microarray and real-time quantitative polymerase chain reaction (qRT-PCR) showed a significant upregulation of lncRNA loc105377478. Functional experiments suggested lncRNA loc105377478 enhanced the expression of IL-6, IL-8 and ROS in NPs-Nd2O3-treated 16HBE cells, and it was further demonstrated that lncRNA loc105377478 promoted the activation of NF-κB by negatively regulating ADIPOR1 expression. Moreover, the expression of IL-6 and IL-8 in NPs-Nd2O3-treated 16HBE cells was regulated by lncRNA loc105377478, which was mediated by the NF-κB signaling pathway. In conclusion, lncRNA loc105377478 promotes NF-κB activation by negatively regulating ADIPOR1 expression, thereby upregulating the expression of IL-6 and IL-8 in 16HBE cells treated with NPs-Nd2O3.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxidos / FN-kappa B / Células Epiteliales / Nanopartículas / Receptores de Adiponectina / ARN Largo no Codificante / Neodimio Límite: Humans Idioma: En Revista: Ecotoxicol Environ Saf Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxidos / FN-kappa B / Células Epiteliales / Nanopartículas / Receptores de Adiponectina / ARN Largo no Codificante / Neodimio Límite: Humans Idioma: En Revista: Ecotoxicol Environ Saf Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos