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Profiling Protein-DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis.
Susila, Hendry; Nasim, Zeeshan; Jin, Suhyun; Youn, Geummin; Jeong, Hyewon; Jung, Ji-Yul; Ahn, Ji Hoon.
Afiliación
  • Susila H; Department of Life Sciences, Korea University, Seoul, South Korea.
  • Nasim Z; Department of Life Sciences, Korea University, Seoul, South Korea.
  • Jin S; Department of Life Sciences, Korea University, Seoul, South Korea.
  • Youn G; Department of Life Sciences, Korea University, Seoul, South Korea.
  • Jeong H; Department of Life Sciences, Korea University, Seoul, South Korea.
  • Jung JY; Department of Life Sciences, Korea University, Seoul, South Korea.
  • Ahn JH; Department of Life Sciences, Korea University, Seoul, South Korea. jahn@korea.ac.kr.
Methods Mol Biol ; 2261: 345-356, 2021.
Article en En | MEDLINE | ID: mdl-33421000
In plant cells, transcription factors play an important role in the regulation of gene expression, which eventually leads to the formation of complex phenotypes. Although chromatin immunoprecipitation (ChIP) involves a lengthy process that requires up to 4 days to complete, it is a powerful technique to investigate the interactions between transcription factors and their target sequences in vivo. Here, we describe a detailed ChIP protocol, focusing on ChIP-qPCR, from material collection to data analyses. Moreover, we explain multiple checkpoints for the quality control of ChIP-qPCR data to ensure the success of this protocol. As this protocol is robust, it can be adapted to other plant materials and plant species, and it can be used for genome-wide profiling experiments, including ChIP-chip and ChIP-seq analyses. We believe that our ChIP-qPCR protocol facilitates research on the interactions between plant transcription factors and their target sequences in vivo.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatina / Reacción en Cadena de la Polimerasa / Arabidopsis / ADN de Plantas / Proteínas de Arabidopsis / Secuenciación de Inmunoprecipitación de Cromatina Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatina / Reacción en Cadena de la Polimerasa / Arabidopsis / ADN de Plantas / Proteínas de Arabidopsis / Secuenciación de Inmunoprecipitación de Cromatina Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos