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Naringin inhibits the biofilms of metallo-ß-lactamases (MßLs) producing Pseudomonas species isolated from camel meat.
Husain, Fohad Mabood; Perveen, Kahkashan; Qais, Faizan Abul; Ahmad, Iqbal; Alfarhan, Ahmed H; El-Sheikh, Mohamed A.
Afiliación
  • Husain FM; Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
  • Perveen K; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
  • Qais FA; Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, India.
  • Ahmad I; Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, India.
  • Alfarhan AH; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
  • El-Sheikh MA; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci ; 28(1): 333-341, 2021 Jan.
Article en En | MEDLINE | ID: mdl-33424314
Food producing animals harbouring bacteria carrying drug resistance genes especially the metallo-beta-lactamase (MBL) pose high risk for the human population. In addition, formation of biofilm by these drug resistant pathogens represents major threat to food safety and public health. In this study, metallo-ß-lactamases (MßLs) producing Pseudomonas spp. from camel meat were isolated and assessed for their biofilm formation. Further, in vitro and in silico studies were performed to study the effect of flavone naringin on biofilm formation against isolated Pseudomonas spp. A total of 55% isolates were found to produce metallo-ß-lactamase enzyme. Naringin mitigated biofilm formation of Pseudomonas isolates up to 57%. Disturbed biofilm architecture and reduced the colonization of bacteria on glass was observed under scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM). The biofilm related traits such as exopolysaccharides (EPS) and alginate production was also reduced remarkably in the presence of naringin. Eradication of preformed biofilms (32-60%) was also observed at the respective 0.50 × MICs. Molecular docking revealed that naringin showed strong affinity towards docked proteins with binding energy ranging from -8.6 to -8.8 kcal mol-1. Presence of metallo-ß-lactamase producers indicates that camel meat could be possible reservoir of drug-resistant Pseudomonas species of clinical importance. Naringin was successful in inhibiting biofilm formation as well as eradicating the preformed biofilms and demonstrated strong binding affinity towards biofilm associated protein. Thus, it is envisaged that naringin could be exploited as food preservative especially against the biofilm forming food-borne Pseudomonas species and is a promising prospect for the treatment of biofilm based infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Saudi J Biol Sci Año: 2021 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Arabia Saudita

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Saudi J Biol Sci Año: 2021 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Arabia Saudita