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Synergistic Effect of Biosynthesized Silver Nanoparticles and Natural Phenolic Compounds against Drug-Resistant Fish Pathogens and Their Cytotoxicity: An In Vitro Study.
Essawy, Ehab; Abdelfattah, Mohamed S; El-Matbouli, Mansour; Saleh, Mona.
Afiliación
  • Essawy E; Clinical Division of Fish Medicine, University of Veterinary Medicine, 1210 Vienna, Austria.
  • Abdelfattah MS; Department of Chemistry, Faculty of Science, Helwan University, Cairo 11795, Egypt.
  • El-Matbouli M; Helwan Nanotechnology Center, Helwan University, Cairo 11795, Egypt.
  • Saleh M; Department of Chemistry, Faculty of Science, Helwan University, Cairo 11795, Egypt.
Mar Drugs ; 19(1)2021 Jan 08.
Artículo en Inglés | MEDLINE | ID: mdl-33429926
ABSTRACT
Fish pathogens causing disease outbreaks represent a major threat to aquaculture industry and food security. The aim of the presented study is to develop safe and effective bioactive agents against two bacterial isolates Aeromonas hydrophila and Pseudomonas fluorescens. We employed a broth microdilution method to investigate the antibacterial effect of biosynthesized silver nanoparticles (AgNPs); rutin, a natural flavonoid extracted from Ruta graveneoles; and heliomycin, a secondary metabolite produced by marine actinomycetes AB5, as monotherapeutic agents. Moreover, AgNPs in combination with rutin (AgNP + R) and heliomycin (AgNPs + H) were examined for their synergistic effect. The cytotoxic effect of individual bioactive compounds and in combination with AgNPs was investigated on epithelioma papulosum cyprini (EPC) fish cell lines. Individual treatment of AgNPs, rutin, and heliomycin exhibited a dose-dependent antimicrobial activity against A. hydrophila and P. fluorescens. Rutin minimum inhibitory concentration (MIC) showed the lowest cytotoxicity when tested on EPC cell lines, while heliomycin MIC was highly cytotoxic. Combined subtherapeutic doses of AgNPs + R and AgNPs + H displayed additive and synergistic effects against A. hydrophila and P. fluorescens, respectively, with improved results and relative safety profile. The study findings demonstrate that a combination of AgNPs and natural bioactive compounds may represent novel therapeutics fighting fish pathogens potentially affecting the fish farming industry.
Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Idioma: Inglés Asunto de la revista: Biologia / Farmacología Año: 2021 Tipo del documento: Artículo País de afiliación: Austria

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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Idioma: Inglés Asunto de la revista: Biologia / Farmacología Año: 2021 Tipo del documento: Artículo País de afiliación: Austria