An optimised MALDI-TOF assay for phosphatidylcholine-specific phospholipase C.
Anal Methods
; 13(4): 491-496, 2021 02 04.
Article
en En
| MEDLINE
| ID: mdl-33432952
ABSTRACT
The Bacillus cereus phosphatidylcholine-specific phospholipase C (PC-PLCBc) is an enzyme that catalyses the hydrolysis of phosphatidylcholines into phosphocholine and 1,2-diacylglycerols. PC-PLCBc has found applications in both the food industry and in medicinal chemistry. Herein, we report our work in the development and optimisation of a matrix assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrometry-based assay to monitor PC-PLCBc activity. The use of one-phase and two-phase reaction systems to assess the inhibition of PC-PLCBc with different structural classes of inhibitors was compared. We also highlighted the advantage of our assay over the commonly used commercially available Amplex Red assay. This method will also be applicable to work on the activity and inhibition of other phospholipases.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatidilcolinas
/
Fosfolipasas de Tipo C
Idioma:
En
Revista:
Anal Methods
Año:
2021
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda