The combined impact of protein corona-free property of starch coated poly (methyl methacrylate) nanoparticles: Amylose content and surface charge.

The protein corona on nano drug carriers is an important well-known biological issue that often induce biological incompatibility and screens the targeting molecules on the surfaces of carriers, therefore, the design of NPs with good protein corona-free property is highly desired and challenged. The natural polysaccharide has been demonstrated as one types of stealth materials after the functional group modification process, but the types and structures of their chains has never been considered. Here, we have designed five types of core-shell starch-coated poly (methyl methacrylate) nanoparticles and we found the starch coated NPs with low amylose content (<15%) could exhibit the excellent protein corona-free property without any modification and the starch with high amylose content coated NPs can also exhibit protein corona-free property after etherifying the surface of NPs to positive surface charge. Therefore, the combined impact of both low amylose content and positive surface charges by etherification modification of the starch can provide the excellent protein corona-free property for starch coated polymer NPs, that is very promising for highly efficient nano drug carries and marine coatings.