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Pyruvate accelerates palladium reduction by regulating catabolism and electron transfer pathway in Shewanella oneidensis.
Cheng, Yuan-Yuan; Wang, Wen-Jing; Ding, Shi-Ting; Zhang, Ming-Xing; Tang, Ai-Guo; Zhang, Ling; Li, Dao-Bo; Li, Bing-Bing; Deng, Guo-Zhi; Wu, Chao.
Afiliación
  • Cheng YY; School of Life Sciences, Anhui University, China.
  • Wang WJ; Information Materials and Intelligent Sensing Laboratory of Anhui Province, Anhui University, China.
  • Ding ST; School of Resources and Environmental Engineering, Anhui University, China.
  • Zhang MX; School of Life Sciences, Anhui University, China.
  • Tang AG; School of Life Sciences, Anhui University, China.
  • Zhang L; School of Life Sciences, Anhui University, China.
  • Li DB; Information Materials and Intelligent Sensing Laboratory of Anhui Province, Anhui University, China.
  • Li BB; School of Resources and Environmental Engineering, Anhui University, China.
  • Deng GZ; Guangdong Institute of Microbiology, Guangdong Province, China.
  • Wu C; School of Environment and Civil Engineering, Jiangnan University, Jiangsu Province, China.
Appl Environ Microbiol ; 87(8)2021 04 15.
Article en En | MEDLINE | ID: mdl-33514518
ABSTRACT
Shewanella oneidensis is a model strain of the electrochemical active bacteria (EAB) because of its strong capability of extracellular electron transfer (EET) and genetic tractability. In this study, we investigated the effect of carbon sources on EET in S. oneidensis by using reduction of palladium ions (Pd(II)) as a model and found that pyruvate greatly accelerated the Pd(II) reduction compared with lactate by resting cells. Both Mtr pathway and hydrogenases played a role in Pd(II) reduction when pyruvate was used as a carbon source. Furthermore, in comparison with lactate-feeding S. oneidensis, the transcriptional levels of formate dehydrogenases involving in pyruvate catabolism, Mtr pathway, and hydrogenases in pyruvate-feeding S. oneidensis were up-regulated. Mechanistically, the enhancement of electron generation from pyruvate catabolism and electron transfer to Pd(II) explains the pyruvate effect on Pd(II) reduction. Interestingly, a 2-h time window is required for pyruvate to regulate transcription of these genes and profoundly improve Pd(II) reduction capability, suggesting a hierarchical regulation for pyruvate sensing and response in S. oneidensis IMPORTANCE The unique respiration of EET is crucial for the biogeochemical cycling of metal elements and diverse applications of EAB. Although a carbon source is a determinant factor of bacterial metabolism, the research into the regulation of carbon source on EET is rare. In this work, we reported the pyruvate-specific regulation and improvement of EET in S. oneidensis and revealed the underlying mechanism, which suggests potential targets to engineer and improve the EET efficiency of this bacterium. This study sheds light on the regulatory role of carbon sources in anaerobic respiration in EAB, providing a way to regulate EET for diverse applications from a novel perspective.

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Appl Environ Microbiol Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Appl Environ Microbiol Año: 2021 Tipo del documento: Article País de afiliación: China