Solubilization of Expressed Proteins from Inclusion Bodies.
Cold Spring Harb Protoc
; 2021(2)2021 02 01.
Article
en En
| MEDLINE
| ID: mdl-33526420
ABSTRACT
The expression of foreign proteins at high levels in Escherichia coli often results in the formation of cytoplasmic granules or inclusion bodies composed of insoluble aggregates of the expressed protein. These inclusion bodies can be seen with a phase-contrast microscope and are readily separated from most soluble and membrane-bound bacterial proteins, as described in this protocol.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes
/
Fraccionamiento Celular
/
Cuerpos de Inclusión
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2021
Tipo del documento:
Article