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Population structure and evolution of resistance to acetolactate synthase (ALS)-inhibitors in Amaranthus tuberculatus in Italy.
Milani, Andrea; Lutz, Ulrich; Galla, Giulio; Scarabel, Laura; Weigel, Detlef; Sattin, Maurizio.
Afiliación
  • Milani A; Institute for Sustainable Plant Protection (IPSP-CNR), Legnaro, Italy.
  • Lutz U; Max Planck Institute for Developmental Biology, Tübingen, Germany.
  • Galla G; Edmund Mach Foundation, S. Michele all'Adige, Italy.
  • Scarabel L; Institute for Sustainable Plant Protection (IPSP-CNR), Legnaro, Italy.
  • Weigel D; Max Planck Institute for Developmental Biology, Tübingen, Germany.
  • Sattin M; Institute for Sustainable Plant Protection (IPSP-CNR), Legnaro, Italy.
Pest Manag Sci ; 77(6): 2971-2980, 2021 Jun.
Article en En | MEDLINE | ID: mdl-33631029
ABSTRACT

BACKGROUND:

Before 2010, Amaranthus tuberculatus (Moq.) J. D. Sauer was barely known to farmers and stakeholders in Italy. Since then, several populations resistant to acetolactate synthase (ALS)-inhibiting herbicides have been collected. In most populations, a known target site resistance-endowing mutation was found, a Trp to Leu substitution at position 574 of the ALS gene, but it was unclear whether they had evolved resistance independently or not. The aims of the work were (i) to elucidate the population structure of Italian ALS-resistant A. tuberculatus populations, and (ii) to analyze the ALS haplotypes of the various populations to determine whether resistance arose multiple times independently.

RESULTS:

In order to determine the population structure of eight A. tuberculatus populations, eight previously described microsatellite loci were used. Two ancestors were found three populations derived from one, and five from the other. In the 4-kb ALS region of the genome, including the 2-kb coding region, 389 single nucleotide polymorphisms were found. In silico haplotype estimation was used to reconstruct the sequence of three distinct haplotypes carrying the Trp574Leu mutation. In addition, no mutation was found in 83% of plants of a single population.

CONCLUSIONS:

(i) Resistance must have arisen independently at least three times; (ii) at least one population was already resistant to ALS inhibitors when introduced in Italy; (iii) a single haplotype with a Trp574Leu mutation was shared among six populations, probably because of broad seed dispersal; and (iv) one population likely evolved nontarget site ALS inhibitors resistance. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetolactato Sintasa / Amaranthus / Herbicidas País/Región como asunto: Europa Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Italia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetolactato Sintasa / Amaranthus / Herbicidas País/Región como asunto: Europa Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Italia