Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.
Int J Biol Macromol
; 178: 170-179, 2021 May 01.
Article
en En
| MEDLINE
| ID: mdl-33639188
ABSTRACT
Iron-enriched Cordyceps militaris was obtained by adding FeSO4 solution to the mycelia for biotransformation. The polysaccharide-iron (III) was extracted by water extraction and alcohol precipitation. High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components. The second component was purified by Sephadex G-150 and named as CPS-iron-II. The average molecular weight of CPS-iron-II was 44.136 kDa. The content of iron was 2.73%. The monosaccharide composition analysis indicated that the CPS-iron-II was composed of rhamnose, arabinose, galactose, glucose, mannose, galacturonic acid with percentage ratio of 0.943.1227.0136.6230.202.12. The results of methylation analysis revealed that the CPS-iron-II was made of â2)-ß-D-Glcp-(1â, with â2, 4)-α-D-Glcp-(1â highly branched. Congo-red test showed that CPS-iron-II can cause flocculation of Congo red solution. The anti-oxidative analysis showed that antioxidant activity of CPS-iron-II was almost equal to that of Vc. The manuscript provided a new way for the preparation of polysaccharide-iron(III) from Cordyceps militaris.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Compuestos Férricos
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Cordyceps
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Antioxidantes
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2021
Tipo del documento:
Article