Protocol for assay of transposase accessible chromatin sequencing in non-model species.
STAR Protoc
; 2(1): 100341, 2021 03 19.
Article
en En
| MEDLINE
| ID: mdl-33659905
ABSTRACT
The assay for transposase accessible chromatin (ATAC-seq) is a method for mapping genome-wide chromatin accessibility. Coupled with high-throughput sequencing, it enables integrative epigenomics analyses. ATAC-seq requires direct access to cell nuclei, a major challenge in non-model species such as small invertebrates, whose soft tissue is surrounded by a protective exoskeleton. Here, we present modifications of the ATAC-seq protocol for applications in small crustaceans, extending applications to non-model species. For complete information on the use and execution of this protocol, please refer to Buenrostro et al. (2013).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatina
/
Mapeo Cromosómico
/
Transposasas
/
Daphnia
/
Secuenciación de Inmunoprecipitación de Cromatina
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido