The function of BAP18 on modulation of androgen receptor action in luteinized granulosa cells from normal weight women with and without PCOS.
Mol Cell Endocrinol
; 527: 111228, 2021 05 01.
Article
en En
| MEDLINE
| ID: mdl-33662476
ABSTRACT
Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in reproductive-age women. In this study, BPTF associated protein of 18 kDa (BAP18) is decreased in luteinized granulosa cells (GCs) from PCOS women. BAP18 depletion significantly decreases CYP19A1 expression levels, leading to an abrogation in transfer capacity of androgen to estrogen in GCs. Also, BAP18 knockdown delays cell cycle G1 to S phase transition and induces cell apoptosis to decrease GCs proliferation. We also provide evidence showing BAP18 interacts with androgen receptor (AR) and enhances AR-mediated transactivation in GCs. Results indicate that AR or BAP18 recruits to androgen response elements (AREs) of CYP19A1 and FSHR, which are putative AR-induced genes in GCs. BAP18 interacts with Sp1 transcription factor and co-recruits to the promoter region of AR gene, resulting in AR transactivation in GCs. Taken together, these data provide new insights on the pathophysiology of PCOS.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Síndrome del Ovario Poliquístico
/
Receptores Androgénicos
/
Fase G1
/
Fase S
/
Proteínas de Unión al ADN
/
Células de la Granulosa
Límite:
Adult
/
Female
/
Humans
Idioma:
En
Revista:
Mol Cell Endocrinol
Año:
2021
Tipo del documento:
Article
País de afiliación:
China