Engineering a heterologous synthetic pathway in Escherichia coli for efficient production of biotin.
Biotechnol Lett
; 43(6): 1221-1228, 2021 Jun.
Article
en En
| MEDLINE
| ID: mdl-33666816
ABSTRACT
OBJECTIVE:
To enhance biotin production in Escherichia coli by engineering a heterologous biotin synthetic pathway.RESULTS:
Biotin operon genes from Pseudomonas putida, which consisted of a bioBFHCD cluster and a bioA gene, was engineered into Escherichia coli for biotin production. The introduction of bioW gene from Bacillus subtilis, encoding pimeloyl-CoA synthetase and sam2 gene from Saccharomyces cerevisiae, encoding S-adenosyl-L-methionine (SAM) synthetase contributed to the heterologous production of biotin in recombinant E. coli. Furthermore, biotin production was efficiently enhanced by optimization of the fermentation compositions, especially pimelic acid and L-methionine, the precursor related to the pimeloyl-CoA and SAM synthesis, respectively. The combination of overexpression of the heterologous biotin operon genes and enhanced supply of key intermediate pimeloyl-CoA and SAM increased biotin production in E. coli by more than 121-fold. With bioprocess engineering efforts, biotin was produced at a final titer of 92.6 mg/L in a shake flask and 208.7 mg/L in a fed-batch fermenter.CONCLUSION:
Through introduction of heterologous biotin synthetic pathway, increasing the supply of precursor pimeloyl-CoA and cofactor SAM can significantly enhance biotin production in E. coli.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Bacillus subtilis
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Biotina
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Pseudomonas putida
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Escherichia coli
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Vías Biosintéticas
Idioma:
En
Revista:
Biotechnol Lett
Año:
2021
Tipo del documento:
Article