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Examination of Novel Immunomodulatory Effects of L-Sulforaphane.
Mazarakis, Nadia; Anderson, Jeremy; Toh, Zheng Quan; Higgins, Rachel A; Do, Lien Anh Ha; Luwor, Rodney B; Snibson, Kenneth J; Karagiannis, Tom C; Licciardi, Paul V.
Afiliación
  • Mazarakis N; Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, VIC 3010, Australia.
  • Anderson J; New Vaccines, Infection and Immunity, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia.
  • Toh ZQ; New Vaccines, Infection and Immunity, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia.
  • Higgins RA; Department of Paediatrics, University of Melbourne, Parkville, VIC 3052, Australia.
  • Do LAH; New Vaccines, Infection and Immunity, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia.
  • Luwor RB; Department of Paediatrics, University of Melbourne, Parkville, VIC 3052, Australia.
  • Snibson KJ; New Vaccines, Infection and Immunity, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia.
  • Karagiannis TC; New Vaccines, Infection and Immunity, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia.
  • Licciardi PV; Department of Paediatrics, University of Melbourne, Parkville, VIC 3052, Australia.
Nutrients ; 13(2)2021 Feb 12.
Article en En | MEDLINE | ID: mdl-33673203
ABSTRACT
The dietary isothiocyanate L-sulforaphane (LSF), derived from cruciferous vegetables, is reported to have several beneficial biological properties, including anti-inflammatory and immunomodulatory effects. However, there is limited data on how LSF modulates these effects in human immune cells. The present study was designed to investigate the immunomodulatory effects of LSF (10 µM and 50 µM) on peripheral blood mononuclear cell (PBMC) populations and cytokine secretion in healthy adult volunteers (n = 14), in the presence or absence of bacterial (lipopolysaccharide) and viral (imiquimod) toll-like receptor (TLRs) stimulations. Here, we found that LSF reduced pro-inflammatory cytokines interleukin (IL)-6, IL-1ß, and chemokines monocyte chemoattractant protein (MCP)-1 irrespective of TLR stimulations. This result was associated with LSF significantly reducing the proportion of natural killer (NK) cells and monocytes while increasing the proportions of dendritic cells (DCs), T cells and B cells. We found a novel effect of LSF in relation to reducing cluster of differentiation (CD) 14+ monocytes while simultaneously increasing monocyte-derived DCs (moDCs lineage-Human Leukocyte Antigen-DR isotype (HLA-DR)+CD11blow-high CD11chigh). LSF was also shown to induce a 3.9-fold increase in the antioxidant response element (ARE) activity in a human monocyte cell line (THP-1). Our results provide important insights into the immunomodulatory effects of LSF, showing in human PBMCs an ability to drive differentiation of monocytes towards an immature monocyte-derived dendritic cell phenotype with potentially important biological functions. These findings provide insights into the potential role of LSF as a novel immunomodulatory drug candidate and supports the need for further preclinical and phase I clinical studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfóxidos / Leucocitos Mononucleares / Isotiocianatos / Inmunomodulación / Factores Inmunológicos Límite: Adult / Female / Humans / Male Idioma: En Revista: Nutrients Año: 2021 Tipo del documento: Article País de afiliación: Australia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfóxidos / Leucocitos Mononucleares / Isotiocianatos / Inmunomodulación / Factores Inmunológicos Límite: Adult / Female / Humans / Male Idioma: En Revista: Nutrients Año: 2021 Tipo del documento: Article País de afiliación: Australia