Role of vitronectin-rich protein corona on tumor-specific siRNA delivery and transfection with lipid nanoparticles.
Nanomedicine (Lond)
; 16(7): 535-551, 2021 03.
Article
en En
| MEDLINE
| ID: mdl-33683145
ABSTRACT
Aim:
To evaluate the role of vitronectin-enriched protein corona on systemic delivery of siRNA-encapsulated cationic lipid nanoparticles (LNPs) to αvß3 integrin expressing solid tumors. Materials &methods:
1,2-Dioleoyl-3-trimethylammonium-propane LNPs were formulated, protein corona formed in nude mice serum and its impact on drug delivery were analyzed.Results:
1,2-Dioleoyl-3-trimethylammonium-propane-containing LNP led to enhanced recruitment of vitronectin and showed preferential transfection to αvß3-expressed cells relative to controls. Upon systemic administration in mice, the LNPs accumulated in the αvß3-expressing endothelial lining of the tumor blood vessels before reaching tumor cells.Conclusion:
These results present an optimized LNP that selectively recruits endogenous proteins in situ to its corona which may lead to enhanced delivery and transfection in tissues of interest.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nanopartículas
Límite:
Animals
Idioma:
En
Revista:
Nanomedicine (Lond)
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM