Enriched environment ameliorates chronic temporal lobe epilepsy-induced behavioral hyperexcitability and restores synaptic plasticity in CA3-CA1 synapses in male Wistar rats.
J Neurosci Res
; 99(6): 1646-1665, 2021 06.
Article
en En
| MEDLINE
| ID: mdl-33713475
ABSTRACT
Temporal lobe epilepsy (TLE) is the most common form of focal epilepsies. Pharmacoresistance and comorbidities pose significant challenges to its treatment necessitating the development of non-pharmacological approaches. In an earlier study, exposure to enriched environment (EE) reduced seizure frequency and duration and ameliorated chronic epilepsy-induced depression in rats. However, the cellular basis of beneficial effects of EE remains unknown. Accordingly, in the current study, we evaluated the effects of EE in chronic epilepsy-induced changes in behavioral hyperexcitability, synaptic transmission, synaptophysin (SYN), and calbindin (CB) expression, hippocampal subfield volumes and cell density in male Wistar rats. Epilepsy was induced by lithium-pilocarpine-induced status epilepticus. Chronic epilepsy resulted in behavioral hyperexcitability, decreased basal synaptic transmission, increased paired-pulse facilitation ratio, decreased hippocampal subfields volumes. Moreover, epileptic rats showed decreased synaptophysin and CB expression in the hippocampus. Six weeks post-SE, epileptic rats were exposed to EE for 2 weeks, 6 hr/day. EE significantly reduced the behavioral hyperexcitability and restored basal synaptic transmission correlating with increased expression of SYN and CB. Our results reaffirm the beneficial effects of EE on behavior in chronic epilepsy and establishes some of the putative cellular mechanisms. Since drug resistance and comorbidities are a major concern in TLE, we propose EE as a potent non-pharmacological treatment modality to mitigate these changes in chronic epilepsy.
Palabras clave
RRID: AB_10679369; RRID: AB_2336830; RRID: AB_258431; RRID: AB_258818; RRID: AB_477523; RRID: SCR_002285; RRID: SCR_005552; RRID: SCR_008590; RRID: SCR_014210; RRID: SCR_016495; RRID: SCR_018946; RRID: SCR_018947; RRID: SCR_018949; RRID: SCR_018956; RRID: SCR_019028; RRID: SCR_019033; RRID: SCR_019036; RRID: SCR_019037; RRID: SCR_019046; RRID: SCR_019047; basal synaptic transmission; behavioral hyperexcitability; enriched environment; hippocampal cell density; long-term potentiation; paired-pulse facilitation; pilocarpine; synaptic plasticity; synaptophysin and calbindin expression; temporal lobe epilepsy
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sinapsis
/
Ambiente
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Epilepsia del Lóbulo Temporal
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Región CA1 Hipocampal
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Región CA3 Hipocampal
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Hipercinesia
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Plasticidad Neuronal
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
India