Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies.
Biochim Biophys Acta Proteins Proteom
; 1869(7): 140653, 2021 07.
Article
en En
| MEDLINE
| ID: mdl-33757896
ABSTRACT
Site-Directed Spin Labelling (SDSL) technique is based on the attachment of a paramagnetic label onto a specific position of a protein (or other bio-molecules) and the subsequent study by Electron Paramagnetic Resonance (EPR) spectroscopy. In particular, continuous-wave EPR (cw-EPR) spectra can detect the local conformational dynamics for proteins under various conditions. Moreover, pulse-EPR experiments on doubly spin-labelled proteins allow measuring distances between spin centres in the 1.5-8 nm range, providing information about structures and functions. This review focuses on SDSL-EPR spectroscopy as a structural biology tool to investigate proteins using nitroxide labels. The versatility of this spectroscopic approach for protein structural characterization has been demonstrated through the choice of recent studies. The main aim is to provide a general overview of the technique, particularly for non-experts, to spread the applicability of this technique in various fields of structural biology.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Marcadores de Spin
/
Proteínas
/
Óxidos de Nitrógeno
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Biochim Biophys Acta Proteins Proteom
Año:
2021
Tipo del documento:
Article
País de afiliación:
Italia