miR1433p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis.
Mol Med Rep
; 23(5)2021 05.
Article
en En
| MEDLINE
| ID: mdl-33760149
ABSTRACT
Endometriosis (EM) is a multifactorial and debilitating chronic benign gynecological disease, but the pathogenesis of the disease is not completely understood. Dysregulated expression of microRNAs (miRNA/miR) is associated with the etiology of EM due to their role in regulating endometrial stromal cell proliferation and invasion. The present study aimed to identify the functions and mechanisms underlying miR1433p in EM. To explore the role of miR1433p in EM, functional miRNAs were analyzed via bioinformatics analysis. miR1433p expression levels in endometriotic stromal cells (ESCs) and normal endometrial stromal cells (NESCs) were measured via reverse transcriptionquantitative PCR. The role of miR1433p in regulating ESC proliferation and invasion was assessed by performing Cell Counting Kit8 and Transwell assays, respectively. miR1433p expression was significantly upregulated in ESCs compared with NESCs. Functionally, miR1433p overexpression inhibited ESC proliferation and invasion, whereas miR1433p knockdown promoted ESC proliferation and invasion. Moreover, miR1433p inhibited autophagy activation in ESCs, as indicated by decreased green puncta, which represented autophagic vacuoles, decreased microtubule associated protein 1 light chain 3α expression and increased p62 expression in the miR1434p mimic group compared with the control group. Moreover, compared with the control group, miR1433p overexpression significantly decreased the expression levels of autophagyrelated 2B (ATG2B), a newly identified target gene of miR1433p, in ESCs. ATG2B overexpression reversed miR1433p overexpressionmediated inhibition of ESC proliferation and invasion. Collectively, the results of the present study suggested that miR1433p inhibited EM progression, thus providing a novel target for the development of therapeutic agents against EM.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Autofagia
/
Proteínas de Transporte Vesicular
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MicroARNs
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Endometriosis
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Proteínas Relacionadas con la Autofagia
Límite:
Adult
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Mol Med Rep
Año:
2021
Tipo del documento:
Article