Quantitative Determination of Methohexital in Human Whole Blood by Liquid Chromatography Tandem Mass Spectrometry.
J Chromatogr Sci
; 59(6): 521-525, 2021 May 20.
Article
en En
| MEDLINE
| ID: mdl-33774658
ABSTRACT
A rapid, simple and sensitive liquid chromatography tandem mass spectrometry method for the determination of methohexital in human whole blood was developed and validated. Ethyl acetate/n-hexane (91) was used as extraction solvent while aprobarbital was used as internal standard. Methohexital was recovered by liquid-liquid extraction from 100 µL of human whole blood. The mobile phase was water-acetonitrile, and an ACQUITY BEH C18 (2.1 × 100 mm, 1.7 µm) column was adopted. Negative electrospray ionization source and multiply reaction monitoring mode were applied. The transitions of m/z were 261.2/42.2 and 261.2/119.0 for methohexital. The limit of detection was 0.5 ng/mL, which was lower than the previous methods. Wide linear range (2-2,000 ng/mL) with a good correlation coefficient (r > 0.99) was also obtained. The intra- and inter- day precisions represented by relative standard deviation were <11.5%, and the recoveries were >79.67%. This analytical method involved small sample volume and had been proven to be rapid, easy, sensitive and specific. Therefore, it could be used for the clinical analysis of methohexital.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Anestésicos Intravenosos
/
Espectrometría de Masas en Tándem
/
Metohexital
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr Sci
Año:
2021
Tipo del documento:
Article
País de afiliación:
China