Low chloride-dependent release of taurine by a furosemide-sensitive process in the in vivo rat hippocampus.
Neuroscience
; 24(3): 885-91, 1988 Mar.
Article
en En
| MEDLINE
| ID: mdl-3380306
ABSTRACT
Extracellular amino acid levels and field potentials evoked by perforant pathway stimuli were studied in vivo by means of a dialysis device, perfusing the rat dentate gyrus with low chloride solutions. When balanced with acetate, these perfusions enhanced the granule cell population spike amplitude. A specific extracellular taurine enhancement occurred whenever Cl- was replaced by acetate solution, reaching an increase of 20-fold over the basal taurine levels when 125 mM Cl- was replaced, whereas other amino acids remained unchanged. A considerable degree of Cl- replacement with iodide was needed, however, to obtain significant increases of extracellular taurine. Perfusions with bromide instead of Cl- did not cause any change in levels of extracellular amino acids including taurine. Furosemide, an inhibitor of Cl- transport, greatly reduced the taurine increase evoked by the low extracellular concentration of permeant anions. This drug also inhibited the taurine release induced by perfusion with 9 mM K+. These findings indicate that the extracellular increase of taurine, evoked by low permeant anion concentrations, may result from the taurine release through a furosemide-sensitive process.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Taurina
/
Cloruros
/
Furosemida
/
Hipocampo
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Neuroscience
Año:
1988
Tipo del documento:
Article
País de afiliación:
España