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Expression of Musashi-1 Increases in Bone Healing.
Padial-Molina, Miguel; Crespo-Lora, Vicente; Candido-Corral, Clara; Martin-Morales, Nati; Abril-Garcia, Dario; Galindo-Moreno, Pablo; Hernandez-Cortes, Pedro; O'Valle, Francisco.
Afiliación
  • Padial-Molina M; Department of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain.
  • Crespo-Lora V; Department of Pathology, University of Granada, 18071 Granada, Spain.
  • Candido-Corral C; Department of Pathology, University of Granada, 18071 Granada, Spain.
  • Martin-Morales N; Department of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain.
  • Abril-Garcia D; Department of Pathology, University of Granada, 18071 Granada, Spain.
  • Galindo-Moreno P; Department of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain.
  • Hernandez-Cortes P; Department of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain.
  • O'Valle F; Department of Orthopedic Surgery, San Cecilio University Hospital, 18071 Granada, Spain.
Int J Mol Sci ; 22(7)2021 Mar 26.
Article en En | MEDLINE | ID: mdl-33810326
ABSTRACT
Musashi-1 (MSI1) is an RNA-binding protein that regulates progenitor cells in adult and developing organisms to maintain self-renewal capacities. The role of musashi-1 in the bone healing environment and its relation with other osteogenic factors is unknown. In the current study, we analyze the expression of MSI1 in an experimental model of rat femoral bone fractures. We also analyze the relation between MSI1 expression and the expression of two osteogenic markers periostin (POSTN) and runt-related transcription factor 2 (RUNX2). We use histological, immunohistochemical, and qPCR techniques to evaluate bone healing and the expression of MSI1, POSTN, and RUNX2 over time (4, 7, and 14 days). We compare our findings with non-fractured controls. We find that in bone calluses, the number of cells expressing MSI1 and RUNX2 increase over time and the intensity of POSTN expression decreases over time. Within bone calluses, we find the presence of MSI1 expression in mesenchymal stromal cells, osteoblasts, and osteocytes but not in hypertrophic chondrocytes. After 14 days, the expression of MSI1, POSTN, and RUNX2 was significantly correlated. Thus, we conclude that musashi-1 potentially serves in the osteogenic differentiation of mesenchymal stromal cells and bone healing. Therefore, further studies are needed to determine the possibility of musashi-1's role as a clinical biomarker of bone healing and therapeutic agent for bone regeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Proteínas de Unión al ARN / Curación de Fractura / Proteínas del Tejido Nervioso Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: España

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Proteínas de Unión al ARN / Curación de Fractura / Proteínas del Tejido Nervioso Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: España