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Recommendations for the introduction of metagenomic next-generation sequencing in clinical virology, part II: bioinformatic analysis and reporting.
de Vries, Jutte J C; Brown, Julianne R; Couto, Natacha; Beer, Martin; Le Mercier, Philippe; Sidorov, Igor; Papa, Anna; Fischer, Nicole; Oude Munnink, Bas B; Rodriquez, Christophe; Zaheri, Maryam; Sayiner, Arzu; Hönemann, Mario; Cataluna, Alba Perez; Carbo, Ellen C; Bachofen, Claudia; Kubacki, Jakub; Schmitz, Dennis; Tsioka, Katerina; Matamoros, Sébastien; Höper, Dirk; Hernandez, Marta; Puchhammer-Stöckl, Elisabeth; Lebrand, Aitana; Huber, Michael; Simmonds, Peter; Claas, Eric C J; López-Labrador, F Xavier.
Afiliación
  • de Vries JJC; Clinical Microbiological Laboratory, department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: jjcdevries@lumc.nl.
  • Brown JR; Microbiology, Virology and Infection Prevention & Control, Great Ormond Street Hospital for Children NHS Foundation Trust, London, United Kingdom. Electronic address: julianne.brown@gosh.nhs.uk.
  • Couto N; Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom. Electronic address: nmgdc20@bath.ac.uk.
  • Beer M; Friedrich-Loeffler-Institute, Institute of Diagnostic Virology, Greifswald, Germany. Electronic address: martin.beer@fli.de.
  • Le Mercier P; Swiss Institute of Bioinformatics, Geneva, Switzerland. Electronic address: Philippe.Lemercier@sib.swiss.
  • Sidorov I; Clinical Microbiological Laboratory, department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: I.A.Sidorov@lumc.nl.
  • Papa A; Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece. Electronic address: annap@auth.gr.
  • Fischer N; University Medical Center Hamburg-Eppendorf, UKE Institute for Medical Microbiology, Virology and Hygiene, Germany. Electronic address: nfischer@uke.de.
  • Oude Munnink BB; Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands. Electronic address: b.oudemunnink@erasmusmc.nl.
  • Rodriquez C; Department of Virology, University hospital Henri Mondor, Assistance Public des Hopitaux de Paris, Créteil, France. Electronic address: christophe.rodriguez@aphp.fr.
  • Zaheri M; Institute of Medical Virology, University of Zurich, Switzerland. Electronic address: zaheri.maryam@virology.uzh.ch.
  • Sayiner A; Dokuz Eylul University, Medical Faculty, Department of Medical Microbiology, Izmir, Turkey. Electronic address: arzu.sayiner@deu.edu.tr.
  • Hönemann M; Institute of Virology, Leipzig University, Leipzig, Germany. Electronic address: Mario.Hoenemann@medizin.uni-leipzig.de.
  • Cataluna AP; Department of Preservation and Food Safety Technologies, IATA-CSIC, Paterna, Valencia, Spain. Electronic address: alba.perez@iata.csic.es.
  • Carbo EC; Clinical Microbiological Laboratory, department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: E.C.Carbo@lumc.nl.
  • Bachofen C; Institute of Virology, University of Zurich, Switzerland. Electronic address: claudia.bachofen@uzh.ch.
  • Kubacki J; Institute of Virology, University of Zurich, Switzerland. Electronic address: jakub.kubacki@uzh.ch.
  • Schmitz D; RIVM National Institute for Public Health and Environment, Bilthoven, the Netherlands. Electronic address: Dennis.Schmitz@RIVM.nl.
  • Tsioka K; Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece. Electronic address: aik.tsioka@gmail.com.
  • Matamoros S; Medical Microbiology and Infection Control, Amsterdam UMC, Amsterdam, the Netherlands. Electronic address: s.p.matamoros@amsterdamumc.nl.
  • Höper D; Friedrich-Loeffler-Institute, Institute of Diagnostic Virology, Greifswald, Germany. Electronic address: dirk.hoeper@fli.de.
  • Hernandez M; Laboratory of Molecular Biology and Microbiology, Instituto Tecnologico Agrario de Castilla y Leon, Valladolid, Spain. Electronic address: hernandez.marta@gmail.com.
  • Puchhammer-Stöckl E; Center of Virology, Medical University Vienna, Vienna, Austria. Electronic address: elisabeth.puchhammer@meduniwien.ac.at.
  • Lebrand A; Swiss Institute of Bioinformatics, Geneva, Switzerland. Electronic address: aitana.lebrand@sib.swiss.
  • Huber M; Institute of Medical Virology, University of Zurich, Switzerland. Electronic address: huber.michael@virology.uzh.ch.
  • Simmonds P; Nuffield Department of Medicine, University of Oxford, Oxford, UK. Electronic address: peter.simmonds@ndm.ox.ac.uk.
  • Claas ECJ; Clinical Microbiological Laboratory, department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: E.C.J.Claas@lumc.nl.
  • López-Labrador FX; Virology Laboratory, Genomics and Health Area, Centre for Public Health Research (FISABIO-Public Health), Valencia, Spain; Department of Microbiology, Medical School, University of Valencia, Spain; CIBERESP, Instituto de Salud Carlos III, Madrid, Spain. Electronic address: F.Xavier.Lopez@uv.es.
J Clin Virol ; 138: 104812, 2021 05.
Article en En | MEDLINE | ID: mdl-33819811
Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety of sample types from patients with infectious syndromes. mNGS is still in its early stages of broader translation into clinical applications. To further support the development, implementation, optimization and standardization of mNGS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mNGS for viral diagnostics to share methodologies and experiences, and to develop application guidelines. Following the ENNGS publication Recommendations for the introduction of mNGS in clinical virology, part I: wet lab procedure in this journal, the current manuscript aims to provide practical recommendations for the bioinformatic analysis of mNGS data and reporting of results to clinicians.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / Biología Computacional Tipo de estudio: Diagnostic_studies / Guideline Límite: Humans Idioma: En Revista: J Clin Virol Asunto de la revista: VIROLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / Biología Computacional Tipo de estudio: Diagnostic_studies / Guideline Límite: Humans Idioma: En Revista: J Clin Virol Asunto de la revista: VIROLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos