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MicroRNA-496 inhibits triple negative breast cancer cell proliferation by targeting Del-1.
Lee, Soo Jung; Jeong, Jae-Hwan; Lee, Jeeyeon; Park, Ho Yong; Jung, Jin Hyang; Kang, Jieun; Kim, Eun Ae; Park, Nora Jee-Young; Park, Ji-Young; Lee, In Hee; Chae, Yee Soo.
Afiliación
  • Lee SJ; Department of Oncology/Hematology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital.
  • Jeong JH; Mmonitor, Inc. 62, Seongseogongdan-ro 11gil, Dalseo-gu.
  • Lee J; Breast & Thyroid Surgery, Kyungpook National University, Kyungpook National University Chilgok Hospital.
  • Park HY; Breast & Thyroid Surgery, Kyungpook National University, Kyungpook National University Chilgok Hospital.
  • Jung JH; Breast & Thyroid Surgery, Kyungpook National University, Kyungpook National University Chilgok Hospital.
  • Kang J; Cell and Matrix Research Institute.
  • Kim EA; Exosome Convergence Research Center, School of Medicine, Kyungpook National University.
  • Park NJ; Pathology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, Republic of Korea.
  • Park JY; Pathology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, Republic of Korea.
  • Lee IH; Department of Oncology/Hematology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital.
  • Chae YS; Department of Oncology/Hematology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital.
Medicine (Baltimore) ; 100(14): e25270, 2021 Apr 09.
Article en En | MEDLINE | ID: mdl-33832090
ABSTRACT
ABSTRACT Del-1 has been linked to the pathogenesis of various cancers, including breast cancer. However, the regulation of Del-1 expression remains unclear. We previously reported the interaction between microRNA-137 (miR-137) and the Del-1 gene. In this study, we investigated miR-496 and miR-137 as regulators of Del-1 expression in triple negative breast cancer (TNBC). Del-1 mRNA and miR-496 were measured by quantitative PCR in breast cancer cells (MDA-MB-231, MCF7, SK-BR3, and T-47D) and tissues from 30 patients with TNBC. The effects of miR-496 on cell proliferation, migration, and invasion were determined with MTT, wound healing, and Matrigel transwell assays, respectively. In MDA-MB-231 cells, miR-496 levels were remarkably low and Del-1 mRNA levels were higher than in other breast cancer cell lines. Luciferase reporter assays revealed that miR-496 binds the 3'-UTR of Del-1 and Del-1 expression is downregulated by miR-496 mimics. Furthermore, miR-496 inhibited the proliferation, migration, and invasion of MDA-MB-231 cells. The effects of miR-496 on cell proliferation were additive with those of miR-137, another miRNA that regulates Del-1 expression. Moreover, in the 30 TNBC specimens, miR-496 was downregulated (P < .005) and the levels of Del-1 in the plasma were significantly elevated as compared with in normal controls (P = .0142). The Cancer Genome Atlas (TCGA) data showed the correlation of miR-496 expression with better overall survival in patients with early TNBC. In in silico and in vitro analyses, we showed that Del-1 is a target of miR-496 in TNBC and thereby affects cancer progression. Our findings suggest that miR-496 and miR-137 additively target Del-1 and act as modulating factors in TNBC. They are potentially new biomarkers for patients with TNBC.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Neoplásica de la Expresión Génica / MicroARNs / Neoplasias de la Mama Triple Negativas Límite: Female / Humans Idioma: En Revista: Medicine (Baltimore) Año: 2021 Tipo del documento: Article Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Neoplásica de la Expresión Génica / MicroARNs / Neoplasias de la Mama Triple Negativas Límite: Female / Humans Idioma: En Revista: Medicine (Baltimore) Año: 2021 Tipo del documento: Article Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA