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The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential.
Onime, Lucy A; Oyama, Linda B; Thomas, Benjamin J; Gani, Jurnorain; Alexander, Peter; Waddams, Kate E; Cookson, Alan; Fernandez-Fuentes, Narcis; Creevey, Christopher J; Huws, Sharon A.
Afiliación
  • Onime LA; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Wales, SY23 3DA, UK.
  • Oyama LB; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, Northern Ireland, BT9 5DL, UK.
  • Thomas BJ; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Wales, SY23 3DA, UK.
  • Gani J; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, Northern Ireland, BT9 5DL, UK.
  • Alexander P; Institute of Infection and Immunity, St. George's University of London, Cranmer Terrace, London, SW17 0RE, UK.
  • Waddams KE; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, Northern Ireland, BT9 5DL, UK.
  • Cookson A; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Wales, SY23 3DA, UK.
  • Fernandez-Fuentes N; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Wales, SY23 3DA, UK.
  • Creevey CJ; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Wales, SY23 3DA, UK.
  • Huws SA; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, Northern Ireland, BT9 5DL, UK.
BMC Microbiol ; 21(1): 105, 2021 04 08.
Article en En | MEDLINE | ID: mdl-33832427
BACKGROUND: The rise of microbial antibiotic resistance is a leading threat to the health of the human population. As such, finding new approaches to tackle these microbes, including development of novel antibiotics is vital. RESULTS: In this study, we mined a rumen eukaryotic metatranscriptomic library for novel Antimicrobial peptides (AMPs) using computational approaches and thereafter characterised the therapeutic potential of the AMPs. We identified a total of 208 potentially novel AMPs from the ruminal eukaryotome, and characterised one of those, namely Lubelisin. Lubelisin (GIVAWFWRLAR) is an α-helical peptide, 11 amino acid long with theoretical molecular weight of 1373.76 D. In the presence of Lubelisin, strains of methicillin-resistant Staphylococcus aureus (MRSA) USA300 and EMRSA-15 were killed within 30 min of exposure with ≥103 and 104 CFU/mL reduction in viable cells respectively. Cytotoxicity of Lubelisin against both human and sheep erythrocytes was low resulting in a therapeutic index of 0.43. Membrane permeabilisation assays using propidium iodide alongside transmission electron microscopy revealed that cytoplasmic membrane damage may contribute to the antimicrobial activities of Lubelisin. CONCLUSIONS: We demonstrate that the rumen eukaryotome is a viable source for the discovery of antimicrobial molecules for the treatment of bacterial infections and further development of these may provide part of the potential solution to the ongoing problem of antimicrobial resistance. The role of these AMPs in the ecological warfare within the rumen is also currently unknown.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rumen / Proteínas Citotóxicas Formadoras de Poros / Staphylococcus aureus Resistente a Meticilina / Eucariontes Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rumen / Proteínas Citotóxicas Formadoras de Poros / Staphylococcus aureus Resistente a Meticilina / Eucariontes Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido