Establishment of primary cell culture of Ruditapes decussatus haemocytes for metal toxicity assessment.
In Vitro Cell Dev Biol Anim
; 57(4): 477-484, 2021 Apr.
Article
en En
| MEDLINE
| ID: mdl-33876341
ABSTRACT
In ecotoxicology, in vitro testing on cell cultures represents an ideal alternative to in vivo strategies for emerging contaminants. These tests have limited use particularly with marine invertebrates like the clams Ruditapes decussatus. In the present study, a primary culture of R. decussatus haemocytes was realized for the first time in order to determine the effect of metals (copper, zinc, and cobalt) on haemocyte parameters like viability and phagocytosis. Results showed that (i) among the studied medium, the modified Leibovitz (L-15) is the best for R. decussatus haemocytes primary culture. (ii) The primary culture system used here represents a suitable in vitro model for assessing cytotoxic responses, (iii) a decrease of cell viability and phagocytosis after 24 h exposure to 100 µg mL-1 CoSO4 and an increase of phagocytosis after 24 h exposure to 50 µg mL-1CuSO4.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Contaminantes Químicos del Agua
/
Bivalvos
/
Hemocitos
/
Metales
Límite:
Animals
/
Humans
Idioma:
En
Revista:
In Vitro Cell Dev Biol Anim
Asunto de la revista:
BIOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Túnez