Your browser doesn't support javascript.
loading
Sinapic Acid Suppresses SARS CoV-2 Replication by Targeting Its Envelope Protein.
Orfali, Raha; Rateb, Mostafa E; Hassan, Hossam M; Alonazi, Mona; Gomaa, Mokhtar R; Mahrous, Noura; GabAllah, Mohamed; Kandeil, Ahmed; Perveen, Shagufta; Abdelmohsen, Usama Ramadan; Sayed, Ahmed M.
Afiliación
  • Orfali R; Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
  • Rateb ME; School of Computing, Engineering & Physical Sciences, University of the West of Scotland, Paisley PA1 2BE, UK.
  • Hassan HM; Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt.
  • Alonazi M; Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62513, Egypt.
  • Gomaa MR; Department of Biochemistry, Faculty of Science, King Saud University. P.O. Box 12372, Riyadh 11495, Saudi Arabia.
  • Mahrous N; Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.
  • GabAllah M; Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.
  • Kandeil A; Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.
  • Perveen S; Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, Giza 12622, Egypt.
  • Abdelmohsen UR; Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
  • Sayed AM; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.
Antibiotics (Basel) ; 10(4)2021 Apr 11.
Article en En | MEDLINE | ID: mdl-33920366
SARS CoV-2 is still considered a global health issue, and its threat keeps growing with the emergence of newly evolved strains. Despite the success in developing some vaccines as a protective measure, finding cost-effective treatments is urgent. Accordingly, we screened a number of phenolic natural compounds for their in vitro anti-SARS CoV-2 activity. We found sinapic acid (SA) selectively inhibited the viral replication in vitro with an half-maximal inhibitory concentration (IC50) value of 2.69 µg/mL with significantly low cytotoxicity (CC50 = 189.3 µg/mL). Subsequently, we virtually screened all currently available molecular targets using a multistep in silico protocol to find out the most probable molecular target that mediates this compound's antiviral activity. As a result, the viral envelope protein (E-protein) was suggested as the most possible hit for SA. Further in-depth molecular dynamic simulation-based investigation revealed the essential structural features of SA antiviral activity and its binding mode with E-protein. The structural and experimental results presented in this study strongly recommend SA as a promising structural motif for anti-SARS CoV-2 agent development.
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Antibiotics (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Antibiotics (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Suiza