Expression cassette and plasmid construction for Yeast Surface Display in Saccharomyces cerevisiae.
Biotechnol Lett
; 43(8): 1649-1657, 2021 Aug.
Article
en En
| MEDLINE
| ID: mdl-33934257
ABSTRACT
OBJECTIVES:
Develop a Cell Surface Display system in Saccharomyces cerevisiae, based on the construction of an expression cassette for pYES2 plasmid.RESULTS:
The construction of an expression cassette containing the α-factor signal peptide and the C-terminal portion of the α-agglutinin protein was made and its sequence inserted into a plasmid named pYES2/gDαAgglutinin. The construction allows surface display of bovine herpesvirus type 5 (BoHV-5) glycoprotein D (gD) on S. cerevisiae BY4741 strain. Recombinant protein expression was confirmed by dot blot, and indirect immunofluorescence using monoclonal anti-histidine antibodies and polyclonal antibodies from mice experimentally vaccinated with a recombinant gD.CONCLUSIONS:
These results demonstrate that the approach and plasmid used represent not only an effective system for immobilizing proteins on the yeast cell surface, as well as a platform for immunobiologicals development.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Saccharomyces cerevisiae
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Proteínas Recombinantes de Fusión
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Técnicas de Visualización de Superficie Celular
Límite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Año:
2021
Tipo del documento:
Article
País de afiliación:
Brasil