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Gene knockout revealed the role of gene feoA in cell growth and division of Lactobacillus delbrueckii subsp. bulgaricus.
Han, Xue; Tu, Yuanqiang; Wu, Huiying; Zhang, Lijuan; Zhao, Sainan.
Afiliación
  • Han X; Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, No.92 Xidazhi Street, Harbin, Heilongjiang, China. xhan@hit.edu.cn.
  • Tu Y; Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, No.92 Xidazhi Street, Harbin, Heilongjiang, China.
  • Wu H; Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, No.92 Xidazhi Street, Harbin, Heilongjiang, China.
  • Zhang L; Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, No.92 Xidazhi Street, Harbin, Heilongjiang, China.
  • Zhao S; Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, No.92 Xidazhi Street, Harbin, Heilongjiang, China.
Arch Microbiol ; 203(6): 3541-3549, 2021 Aug.
Article en En | MEDLINE | ID: mdl-33942158
ABSTRACT
Gene feoA plays an important role in cell growth because of its function of transport Fe2+ which is a necessary element for cells. In this study, the recombinant plasmid pUC19-feoA-Tet was successfully constructed using the inserted gene inactivation method. Using the homologous recombination technique, the tet gene was used as a resistance screening marker to knock out the feoA gene of Lactobacillus delbrueckii subsp. bulgaricus 34.5 (strain 34.5). Comparative analysis of growth curves revealed the growth changes in the absence of feoA gene in strain 34.5. The results showed that the growth of the bacteria was prolonged by 2 h and could be restored in the stationary phase. To further study whether feoA is related to the cell division of strain 34.5, the qPCR experiment was carried out. The results showed that, compared with the wild-type strain, the expression of genes related to cell division in the mutant strain was up-regulated in the pre-log phase, down-regulated in the late-log phase, and returned to the original level in the stationary phase. These findings provide ideas for Lactobacillus delbrueckii subsp. bulgaricus to control division and cell cycle.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Bacteriana de la Expresión Génica / Ciclo Celular / Proteínas de Unión a Hierro / Lactobacillus delbrueckii Idioma: En Revista: Arch Microbiol Año: 2021 Tipo del documento: Article País de afiliación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Bacteriana de la Expresión Génica / Ciclo Celular / Proteínas de Unión a Hierro / Lactobacillus delbrueckii Idioma: En Revista: Arch Microbiol Año: 2021 Tipo del documento: Article País de afiliación: China