YbeY is required for ribosome small subunit assembly and tRNA processing in human mitochondria.
Nucleic Acids Res
; 49(10): 5798-5812, 2021 06 04.
Article
en En
| MEDLINE
| ID: mdl-34037799
ABSTRACT
Mitochondria contain their own translation apparatus which enables them to produce the polypeptides encoded in their genome. The mitochondrially-encoded RNA components of the mitochondrial ribosome require various post-transcriptional processing steps. Additional protein factors are required to facilitate the biogenesis of the functional mitoribosome. We have characterized a mitochondrially-localized protein, YbeY, which interacts with the assembling mitoribosome through the small subunit. Loss of YbeY leads to a severe reduction in mitochondrial translation and a loss of cell viability, associated with less accurate mitochondrial tRNASer(AGY) processing from the primary transcript and a defect in the maturation of the mitoribosomal small subunit. Our results suggest that YbeY performs a dual, likely independent, function in mitochondria being involved in precursor RNA processing and mitoribosome biogenesis. Issue Section Nucleic Acid Enzymes.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ribonucleasas
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ARN de Transferencia
/
Procesamiento Postranscripcional del ARN
/
Proteínas Mitocondriales
/
Subunidades Ribosómicas Pequeñas
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Ribosomas Mitocondriales
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Mitocondrias
Límite:
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido