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Gold nanoparticles and tilt pairs to assess protein flexibility by cryo-electron microscopy.
Jagota, Milind; Townshend, Raphael J L; Kang, Lin-Woo; Bushnell, David A; Dror, Ron O; Kornberg, Roger D; Azubel, Maia.
Afiliación
  • Jagota M; Department of Computer Science, Stanford University, Stanford, CA, USA; Department of Electrical Engineering, Stanford University, Stanford, CA, USA.
  • Townshend RJL; Department of Computer Science, Stanford University, Stanford, CA, USA.
  • Kang LW; Department of Biological Sciences, Konkuk University, Seoul, Korea.
  • Bushnell DA; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA.
  • Dror RO; Department of Computer Science, Stanford University, Stanford, CA, USA; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA; Institute for Computational
  • Kornberg RD; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA.
  • Azubel M; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA. Electronic address: mazubel@stanford.edu.
Ultramicroscopy ; 227: 113302, 2021 08.
Article en En | MEDLINE | ID: mdl-34062386
A computational method was developed to recover the three-dimensional coordinates of gold nanoparticles specifically attached to a protein complex from tilt-pair images collected by electron microscopy. The program was tested on a simulated dataset and applied to a real dataset comprising tilt-pair images recorded by cryo electron microscopy of RNA polymerase II in a complex with four gold-labeled single-chain antibody fragments. The positions of the gold nanoparticles were determined, and comparison of the coordinates among the tetrameric particles revealed the range of motion within the protein complexes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / ARN Polimerasa II / Fragmentos de Inmunoglobulinas / Nanopartículas del Metal / Oro Idioma: En Revista: Ultramicroscopy Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / ARN Polimerasa II / Fragmentos de Inmunoglobulinas / Nanopartículas del Metal / Oro Idioma: En Revista: Ultramicroscopy Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos