[The Role of Zyxin in Regulating Platelet Cytoskeleton Distribution].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 29(3): 876-880, 2021 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-34105487
ABSTRACT
OBJECTIVE:
To investigate the regulatory effect of zyxin on the distribution of platelet cytoskeleton.METHODS:
Platelets were isolated from zyxin-knockout (Zyx-/-) and wild type (WT) mice respectively and corresponding platelet cytoskeleton components were separated. The expressions of ß-actin, α-actinin, filamin A and myosin â ¡A in cytoskeleton components were detected by Western blot. Actin polymerization was induced by the actin polymerization inducer Jasplakinolide (Jas) in WT and Zyx-/- platelets. Also, the expressions of the cytoskeleton proteins in cytoskeleton components were detected by Western blot. WT and Zyx-/- platelets were allowed to spread on fibrinogen-coated surface. Platelet F-actin was labeled with Alexa Fluor 488-conjugated phalloidin and the fluorescent intensity was compared between WT and Zyx-/- platelets.RESULTS:
After zyxin gene was knockout, the expressions of cytoskeleton proteins ß-actin, α-actinin, filamin A, and myosin â ¡ A in resting and Jas-induced platelets were significantly increased. In the platelet spreading on fibrinogen surface, F-actin was increased in Zyx-/- platelets as compared with that in WT platelets.CONCLUSION:
Zyxin significantly regulates the distribution of platelet cytoskeleton, which plays an important role in maintaining platelet cytoskeleton homeostasis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plaquetas
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Citoesqueleto
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Asunto de la revista:
HEMATOLOGIA
Año:
2021
Tipo del documento:
Article