Generation of human induced pluripotent stem cell lines from 2 patients with MIRAGE syndrome.
Stem Cell Res
; 54: 102417, 2021 07.
Article
en En
| MEDLINE
| ID: mdl-34119956
ABSTRACT
MIRAGE syndrome is a multisystem disorder caused by mutations in SAMD9 (sterile α motif domain-containing protein 9) with a high mortality in the first decade of life. We generated 2 human induced pluripotent stem cell lines from male children diagnosed with MIRAGE syndrome. The cell lines were generated from fibroblasts by integration-free reprogramming using the Sendai virus. Both cell lines were fully characterized regarding their pluripotent identity and differentiation potential, and quality controlled for karyotypic integrity, cell line identity and clearance of reprogramming vectors. The generated cell lines represent a valuable tool to study the disease mechanism of MIRAGE syndrome.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre Pluripotentes Inducidas
Límite:
Child
/
Humans
/
Male
Idioma:
En
Revista:
Stem Cell Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
Alemania