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Differential activation of neuroinflammatory pathways in children with seizures: A cross-sectional study.
Bartolini, Luca; Moran, Michael P; Norato, Gina; Thomas, Bobbe; Dick, Alexander D; Wells, Elizabeth; Suslovic, William; Bumbut, Adrian; Chamberlain, James M; Theodore, William H; Gaillard, William D; Jacobson, Steven.
Afiliación
  • Bartolini L; The Warren Alpert Medical School of Brown University, Hasbro Children's Hospital, Providence, RI, United States. Electronic address: luca_bartolini@brown.edu.
  • Moran MP; Division of Neuroimmunology and Neurovirology, NINDS, NIH, Bethesda, MD, United States.
  • Norato G; Office of Biostatistics, NINDS, NIH, Bethesda, MD, United States.
  • Thomas B; Emergency Medicine and Trauma Services, Children's National Medical Center, Washington DC, United States.
  • Dick AD; Center for Neuroscience, Children's National Medical Center/The George Washington University, Washington DC, United States.
  • Wells E; Center for Neuroscience, Children's National Medical Center/The George Washington University, Washington DC, United States.
  • Suslovic W; Center for Neuroscience, Children's National Medical Center/The George Washington University, Washington DC, United States.
  • Bumbut A; Center for Neuroscience, Children's National Medical Center/The George Washington University, Washington DC, United States.
  • Chamberlain JM; Emergency Medicine and Trauma Services, Children's National Medical Center, Washington DC, United States.
  • Theodore WH; Clinical Epilepsy Section, NINDS, NIH, Bethesda, MD, United States.
  • Gaillard WD; Center for Neuroscience, Children's National Medical Center/The George Washington University, Washington DC, United States.
  • Jacobson S; Division of Neuroimmunology and Neurovirology, NINDS, NIH, Bethesda, MD, United States.
Seizure ; 91: 150-158, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34161903
PURPOSE: Inflammation plays a crucial role in epileptogenesis. We analyzed inflammatory cytokines in plasma and saliva from children with seizures and healthy controls and measured their associations with HHV6 and EBV infection. METHODS: We analyzed plasma from 36 children within 24 h of seizures (cases) and 43 healthy controls and saliva from 44 cases and 44 controls with a multiplex immunoassay. Saliva from all controls and 65 cases and blood from 26 controls and 35 cases were also analyzed by PCR for viral DNA. Primary outcome was cytokine levels in cases vs. controls. Secondary outcomes included detection of HHV-6 and EBV viral DNA in cases vs. controls and viral loads in cases vs. controls. Statistical analysis included the Wilcoxon Rank Sum test, Fisher's exact test, ANOVA, and Spearman correlation. RESULTS: Compared to controls, patients had higher levels of CCL11 (p = 0.0018), CCL26 (p<0.001), IL10 (p = 0.044), IL6 (p<0.001), IL8 (p = 0.018), and MIP1ß (p = 0.0012). CCL11 was higher with 3 or more seizures (p = 0.01), seizures longer than 10 min (p = 0.001), and when EEG showed focal slowing (p = 0.02). In saliva, febrile seizures had higher levels of IL-1ß (n = 7, p = 0.04) and new onset seizures had higher IL-6 (n = 15, p = 0.02). Plasma and saliva cytokine levels did not show a correlation. The frequency of HHV-6 and EBV detection was similar across groups and not different than controls. We found no correlation between viral load and cytokine levels. CONCLUSIONS: We showed differential activation of neuroinflammatory pathways in plasma from different seizure etiologies compared to controls, unrelated to viral infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Herpesvirus Humano 6 / Convulsiones Febriles Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Child / Humans Idioma: En Revista: Seizure Asunto de la revista: NEUROLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Herpesvirus Humano 6 / Convulsiones Febriles Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Child / Humans Idioma: En Revista: Seizure Asunto de la revista: NEUROLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido